MmuEX6066474 @ mm10
Exon Skipping
Gene
ENSMUSG00000032289 | Thsd4
Description
thrombospondin, type I, domain containing 4 [Source:MGI Symbol;Acc:MGI:2672033]
Coordinates
chr9:59997006-60003026:-
Coord C1 exon
chr9:60002851-60003026
Coord A exon
chr9:59999941-60000037
Coord C2 exon
chr9:59997006-59997281
Length
97 bp
Sequences
Splice sites
3' ss Seq
CATAGACTTTGTGTCCTCAGATG
3' ss Score
8.15
5' ss Seq
CAGGTAAAG
5' ss Score
9.65
Exon sequences
Seq C1 exon
CCTTGCGAAGTCGCTCTGGCCGCTCCATCATCAATGGGAACTGGGCAATCGATCGCCCGGGGAAGTACGAGGGCGGAGGGACAATGTTCACCTACAAACGACCGAACGAGGTCTCCAGCACTGCGGGGGAGTCCTTTCTGGCTGAGGGTCCCACTAATGAGATCCTGGATGTCTAT
Seq A exon
ATGATACACCAGCAGCCCAACCCAGGAGTCCACTATGAGTACGTGATCATGAGGAATAATGCCATCAGCCCACAGGTGCCACCACACAGGAGACCAG
Seq C2 exon
GAGAACCTTTCAATGGGCAGCTGGAAGAGGAAGACAGAGGCCAGGAGGACAGAGAAGAGAGGGAGAAGAACCAGGAAAAGGAAGACTCACAAGTCGAGGCCCCTGAGGTATTCACGTCAGAATCCACGCAGACCTTCCCAGTCAGGCATCCGGAGAGATTCCCTTCCCACCGACCAGACAACTTGGTGCCCCCAGCACCACAGCCCCCAAGGCGAAGCCGGGACCACAACTGGAAGCAGCTGGGGACAACAGAATGCTCCACCACCTGTGGGAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000032289-'28-35,'28-34,30-35=AN
Average complexity
A_C1
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.390 A=0.818 C2=0.849
Domain overlap (PFAM):
C1:
PF059869=ADAM_spacer1=FE(49.6=100)
A:
PF059869=ADAM_spacer1=PD(13.7=48.5)
C2:
NO
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAATGGGAACTGGGCAATCGA
R:
TTCCAGCTGCCCATTGAAAGG
Band lengths:
171-268
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types