MmuEX6068701 @ mm10
Exon Skipping
Gene
ENSMUSG00000034684 | Sema3f
Description
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F [Source:MGI Symbol;Acc:MGI:1096347]
Coordinates
chr9:107687490-107688088:-
Coord C1 exon
chr9:107687949-107688088
Coord A exon
chr9:107687730-107687844
Coord C2 exon
chr9:107687490-107687559
Length
115 bp
Sequences
Splice sites
3' ss Seq
CTTTCTGCCCCCACCCCCAGAAC
3' ss Score
10.88
5' ss Seq
TCCGTGAGT
5' ss Score
8.93
Exon sequences
Seq C1 exon
ATCCTTCCTTCATACATGCTGAGCTCATCCCTGACAGTGCAGAGCGCAATGACGATAAACTCTACTTCTTCTTCCGAGAGCGCTCGGCAGAGGCGCCACAGAACCCTGCTGTGTATGCCCGCATCGGGCGCATCTGCCTC
Seq A exon
AACGATGACGGTGGCCATTGCTGCCTGGTCAACAAGTGGAGCACATTCCTGAAGGCTCGGCTCGTCTGCTCCGTGCCAGGCGAGGATGGTATTGAGACCCACTTCGACGAACTCC
Seq C2 exon
AGGATGTGTTTGTCCAGCAGACCCAGGACGTCAGGAACCCAGTCATTTATGCTGTCTTTACCTCTTCAGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000034684-'29-26,'29-25,32-26=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.122 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0140314=Sema=FE(10.4=100)
A:
PF0140314=Sema=FE(8.6=100)
C2:
PF0140314=Sema=FE(5.2=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATCCTTCCTTCATACATGCTGAGC
R:
CCTGAAGAGGTAAAGACAGCA
Band lengths:
210-325
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types