Special

MmuINT0012275 @ mm9

Intron Retention

Gene
ENSMUSG00000030609 | Aen
Description
apoptosis enhancing nuclease [Source:MGI Symbol;Acc:MGI:1915298]
Coordinates
chr7:86050729-86053719:+
Coord C1 exon
chr7:86050729-86050929
Coord A exon
chr7:86050930-86052029
Coord C2 exon
chr7:86052030-86053719
Length
1100 bp
Sequences
Splice sites
5' ss Seq
CAGGTATGT
5' ss Score
9.8
3' ss Seq
GTTTTTGCTCCTGTTCACAGGTC
3' ss Score
10.97
Exon sequences
Seq C1 exon
ATCCTTAAGCTGTTGAAGGGCAAGGTGGTGGTGGGGCACGCACTGCACAATGACTTCCAGGCCCTGAAGTACGTCCACCCTCGGAGCCAGACCCGGGATACCACTTATGTCCCAAACCTGCTCAGCCAGCCCAGCTCCCTTATCCGAACTCGGGTCTCTCTTAAGGACCTGGCCTTGAACCTGCTGCACAAGAAGATCCAG
Seq A exon
GTATGTGGTATGGAACCTCTTGGGAGGGAGGGGGCTCATCGGATTTGCATAATTGCTGAGGTGTGGATGGCACTGTGGCCTGCTGCATGCATATAGGCAGTATGGGTGTAGCTATCTCATTTTATTCTCTGTGAGTTCCCCAGTGAAAGTCAAGTTCTTCTAGGCTATATCTCTTTAGGTCTTTGCCCACCTCGCTCCTGCCCAGATCCTATCCCCATAGAATGGATTCCGACTCCCTAGCTCTGGAGGCTCAGGTCAAGGGGCCTTTGACTCTTGCTCTAAGCTATTTTGGTATCCATATTCTAACCTCTGGAGCGGGGATTTTATTTTTTTGGTTTTGTTTTGTTTTGTTTTTCAAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGACTGGACTCTACCCCAGAGGTCTTCCTGCCTCTGCCTCTGCCTCTGCCTCCTGAGTTTTGGGATTGAAGGCATGTGCCACCACAGCCCAGATTCTGGAATTGTTTTTTTAAAATATGTGTAGGTGTTTGGCCTGCACACATGTCTGTGCATCACATGCATGACTCGTTCTTGGAGATCAAAAGAAGGCATCGGATCCTCTGGGACTGGGTTACAGACAGTTTGTGAGCTACCACGTAGGTACTGGGAACTGAGCCTGGGTCCTGGGGAAGAGCAGCCAGTGCTCTTAACCACGGTGCGTTCTCTCCAACCCAACACTAAGGAAGGATTATTTTGCTTCATGGTTTTGTAGGGTCCGTGTGCTTAGGCAGACCATCATGGTGGTAGAAGCATGTGGAGCTATAACCCCACAGTGAAAATGGGGCAGACAAAAGAACAGAGACAGTATATTCCCAAGGACCTTCCCTGGCAACGTGTTTCCTTAGGTCCCTCCTCAGTTGAGTACCTTTCAAGATAGCACTGTGAGCTGGGAACAAGCCCACAGCACACAGACCTTGGAGTATATGTATCTAAATCACAACAGCCTCCATTCAGAGATAAGTGCTCCTGACCCTCCGAGGCTGGCTTTGTTTCAGGGGTGATAGTCAGGCCCATTTCACTACGTAGTGATGTTTTTGCTCCTGTTCACAG
Seq C2 exon
GTCGGTCACCAGGGCCACTCATCTGTGGAAGATGCCATGACAGCCATGGAGTTGTACCAGCTGGTGGAGGTGCAGTGGGAGCAGCAAGTGGCCAGCTCTGCCCAGGCCCCTGCTGAGGACAGGGGGCCCGACAGCAGCACTGACGTGGAGCAGTACATGGACGACCAGTACTGGCCCGAGGACCTGACCCAGAGCACCGGGGCCGAGACAAATGGAGGCCCAGATAGACAGGAGGGAGAGGAAGGGCAGGGAGCCAGGAGTGCTCCTCCCTAGTCACGGGGTAGGATTGGAGTTCTAAGGCAGTGTTTCTCAAAACCTGAGGGTCAGGACTCCTTTATGGGTTTAAAGCTTTTCACAGGGGTCGCCTAAGACCATCGGAAGACACAGCTGTGTACGTTATGATTCATAACTAGCAAAATTAGAGTTATGAAGTAGCAACAGAAATAATTTTATTTTTTGGGGGGGGTCTCCACAGCATGAGGAACTGTAATAAAGGGTTGCAGCATTAGGAAGGTGGAAAGCCACTGGTCTGGGGTAACTTAAGGGCACTGGTAGGCAAAGGCTCAGGTCACACCACCCTCTGACAGTCTCATCATTCCTGACACTTAACATGGAGACACTAGACAAGCCTGTGGCTTTCCCAGTGTAAAGTGGGAAGAGTGGCAGAGCCTACCTCATGGGGTGTGTCTTAGCACTCTGAGTGTATCCTAGTGATAAACAGGATGGGGCTCTGTTCAGGCCTGGTTTCTGAGCAGCTATTGCTCAGGTAGGTCTCTTGGCTTTTCCATCGGATGAGGTGGGGCCCAGGGCAAGGGTATTGCAGGGGAGTTCAGAGGTCTTGCTGCCAGCTTCTATCTCCACATCAGCCTCCTGACACTGCATTTGGAAACGTGACAACAGTAATCCAGGTCAGAAATGAAGGGATGCTACTGAGACCACACTTCCCACTGTCCACTGATTACAGAAAGCGTTAGTTAGTCACGGATTGTTTGATTTCTGGCTCCTCCCTGCCAGGGAAAGGGGGCTGGCCAAATCATCACCACTACTTCTGGCCTGGCACCAGGGTGAGCTCTTGTTTCCTTAAAAAGCAGCCACTTGCGCCAGCCTTGATACTGGGCAGTAGCATTGGTGGGTTGAAGGGTGAAAGAACTGTAAGCTGGGAGAGGCTTACTGGGAGAAGTCAGCTTGCTCATAGTACCAGACCATCGGCATAGGAATAAAGTGCATCTGGAAAAGTGAGTGAACTGGTCCTTTCTGGATGCTCAAGCTGGGCTGAAGGTGTGTGAGGCCAGGGAAGGCCCAGGGCTCTGAGCCTTGCTGGATGCTCGTCTTGAGATGCATTTCAAGATGGTACACGTTAGCTCTACAGCAGTTTCTGACTTCAGTTGGAGTTGGCTATTACACAGCACACTGTTTGATTTGGTCTCTCCCCAAAGCTAGGCTTTCACATTAGAAAGTGTTTGGTTTTTTGCCATTGAAACATAATCTCATGGTAGAGTCCAGCTTCTGTAACTCCTTATGGAACTCAGGCGATCCTCCTGCCTTAGCCCACCCAGGGTTGGCATTGTGAGCATATACTGTCTTTCCTGTAAGACTGCCCTTTAGGACTAAGTCCTTTGTGTATAATGTTAGTCACACGTTCGGTCACTTCCTCTCTTGCTATTGTGAATAAGATAGCCGTATATATGGT
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000030609-Aen:NM_026531:3
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact





	

	
Alternative protein isoforms





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.063 A=NA C2=0.749
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0092919=RNase_T=FE(42.3=100)

							
A:
NA

							
C2:
PF0092919=RNase_T=PD(11.5=19.8)

						

					

				








    
Main Inclusion Isoform:
NA





     
                   









    
Main Skipping Isoform:
ENSMUSP00000103046





     
      









    
Other Inclusion Isoforms:
NA
          









    
Other Skipping Isoforms:
ENSMUSP00000103044, ENSMUSP00000103048
  



Associated events






Other assemblies


mm10




Conservation


Human
(hg38)
chr15:88629427-88630057 
ALTERNATIVE
HsaINT0005078
(AEN)
Human
(hg19)
chr15:89172658-89173288 
ALTERNATIVE
HsaINT0005078
(AEN)
Rat
(rn6)
chr1:140591277-140592454 
LOW PSI
RnoINT0014676
(Aen)
Cow
(bosTau6)
chr21:20006308-20007130 
LOW PSI
BtaINT0015427
(AEN)
Chicken
(galGal4)
chr10:12624666-12624986 
ALTERNATIVE
GgaINT0027090
(AEN)
Chicken
(galGal3)
chr10:14820407-14820727 
LOW PSI
GgaINT0027090
(AEN)
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
AAGCTGTTGAAGGGCAAGGTG

				
R: 
TCCATGTACTGCTCCACGTCA

				
Band lengths: 
356-1456

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






Retrieved from "https://vastdb.crg.eu/wiki/Special:VastDB"