Special

MmuINT0015153 @ mm10

Intron Retention

Gene
ENSMUSG00000069601 | Ank3
Description
ankyrin 3, epithelial [Source:MGI Symbol;Acc:MGI:88026]
Coordinates
chr10:69808416-69808971:+
Coord C1 exon
chr10:69808416-69808517
Coord A exon
chr10:69808518-69808872
Coord C2 exon
chr10:69808873-69808971
Length
355 bp
Sequences
Splice sites
5' ss Seq
CAGGTCCGT
5' ss Score
6.47
3' ss Seq
CTCCTTTCTCTTCCCATCAGAAT
3' ss Score
9.93
Exon sequences
Seq C1 exon
TCTGATGCCAACGCAAGTTACTTAAGAGCAGCTCGGGCAGGGCACCTGGAAAAGGCCCTTGACTACATCAAAAATGGAGTGGACGTCAACATCTGTAACCAG
Seq A exon
GTCCGTTGTGGTTATTGTTACAGTTTGCTATTTTTGTTAAAACGAGTAGATTCCTTAAAGCTTCCCTTAGCTATTTGGCAAGATCTCTCTCCACTTGTCAGGAGGTTGGTTTCGTTGTTAAATTTTGTCAGCCCAATGTTATCCGATTAGTCTCCATCAAAGGGATGCCATTTGGTGACCAATGGCTGATAGTAATGGACCACCCTCCCTAGTTCTCACATTTTATTTCTGTTCCACATTCCTGTGCTACATTCACATGGGGGACTCCGGTAGGGGGCTAACTGAGGAGCAGGTGGCAGGCTGTGGACTATTGAGAACTCTGTTTTCAGTTCACTCTCCTTTCTCTTCCCATCAG
Seq C2 exon
AATGGATTGAATGCACTCCATCTTGCTTCCAAAGAAGGCCATGTGGAAGTGGTCTCTGAGCTGCTGCAGAGGGAAGCCAATGTTGATGCCGCCACAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000069601:ENSMUST00000182884:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact





	

	
ORF disruption upon sequence inclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.228 A=NA C2=0.095
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF127962=Ank_2=PU(28.3=76.5)

							
A:
NA

							
C2:
PF138571=Ank_5=PD(22.2=24.2),PF127962=Ank_2=PU(16.5=39.4)

						

					

				








    
Main Inclusion Isoform:
NA





     
                   









    
Main Skipping Isoform:
ENSMUSP00000061698





     
      









    
Other Inclusion Isoforms:
NA
          









    
Other Skipping Isoforms:
ENSMUSP00000090087, ENSMUSP00000090088, ENSMUSP00000090090, ENSMUSP00000138326, ENSMUSP00000138347, ENSMUSP00000138348, ENSMUSP00000138474, ENSMUSP00000138601, ENSMUSP00000138603, ENSMUSP00000138686, ENSMUSP00000138770, ENSMUSP00000151906
  



Associated events






Other assemblies


mm9




Conservation


Human
(hg38)
chr10:60279149-60279537 
LOW PSI
HsaINT0008155
(ANK3)
Human
(hg19)
chr10:62038907-62039295 
LOW PSI
HsaINT0008155
(ANK3)
Rat
(rn6)
chr20:20259365-20259795 
LOW PSI
RnoINT0017394
(Ank3)
Cow
(bosTau6)
chr28:16072681-16073091 
Chicken
(galGal4)
chr6:8374291-8374776 
LOW PSI
GgaINT0041339
(chAnk3)
Chicken
(galGal3)
chr6:10035201-10035686 
LOW PSI
GgaINT0041339
(Q90717_CHICK)
Zebrafish
(danRer10)
chr12:7771107-7771755 
LOW PSI
DreINT0029768
(ank3b)
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
CGCAAGTTACTTAAGAGCAGCT

				
R: 
CTTTGTGGCGGCATCAACATT

				
Band lengths: 
190-545

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Ribosome-engaged transcriptomes of neuronal types
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types





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