MmuINT0087321 @ mm10
Intron Retention
Gene
ENSMUSG00000006740 | Kif5b
Description
kinesin family member 5B [Source:MGI Symbol;Acc:MGI:1098268]
Coordinates
chr18:6210700-6211095:-
Coord C1 exon
chr18:6211024-6211095
Coord A exon
chr18:6210805-6211023
Coord C2 exon
chr18:6210700-6210804
Length
219 bp
Sequences
Splice sites
5' ss Seq
AAGGTGATC
5' ss Score
4.41
3' ss Seq
CTTTGTCTCTGTTCTCTCAGAGC
3' ss Score
10.21
Exon sequences
Seq C1 exon
GCAAAAGAACTTCAGACTTTACACAACCTGCGTAAGCTCTTTGTTCAGGACTTGGCTACCAGGGTGAAAAAG
Seq A exon
GTGATCTCACAGTGCCCAGGGCTGCTTGTACAAGTGGTGCTTTGCTGTGTGTGGTGCTTTAAGAGCACATATGTGGTCATGTCCACGTGAGTCTGTGATGCCCGTGTTTGTGTGGAGGAACGTGGGTCCTCTGATCCTTAACACTGCTCACTGCTTTGTCTCTTTGTCTCTGACCCTCACACTGCTCACTGCCTTGTCTCTTTGTCTCTGTTCTCTCAG
Seq C2 exon
AGCGCCGAGGTCGACTCTGACGACACTGGCGGCAGTGCTGCACAGAAGCAGAAAATCTCCTTCCTTGAAAACAACCTTGAACAGCTCACCAAAGTGCACAAGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000006740:ENSMUST00000025083:22
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.714
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGAACTTCAGACTTTACACAACCTGC
R:
CTGCTTGTGCACTTTGGTGAG
Band lengths:
172-391
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types