Special

MmuINT0095034 @ mm9

Intron Retention

Gene
ENSMUSG00000002948 | Map2k7
Description
mitogen-activated protein kinase kinase 7 [Source:MGI Symbol;Acc:MGI:1346871]
Coordinates
chr8:4244695-4245003:+
Coord C1 exon
chr8:4244695-4244775
Coord A exon
chr8:4244776-4244860
Coord C2 exon
chr8:4244861-4245003
Length
85 bp
Sequences
Splice sites
5' ss Seq
CTGGTTAGT
5' ss Score
6.32
3' ss Seq
AATGCCTGTCACCCTCCCAGGTG
3' ss Score
9.05
Exon sequences
Seq C1 exon
CCCGAGCGCATCGACCCTCCAGATCCCACCAAGCCTGACTATGACATCCGAGCTGATGTGTGGAGCCTGGGCATCTCACTG
Seq A exon
GTTAGTTGGGGCTGCTGTTCTAGGGCTTGGTGGGAAGGCAGGGATCTCTTACCTGCTGTTTCAGCAATGCCTGTCACCCTCCCAG
Seq C2 exon
GTGGAGCTGGCAACAGGACAGTTCCCCTATAAGAACTGCAAGACGGACTTTGAGGTCCTCACCAAAGTCCTACAGGAAGAGCCCCCACTCCTGCCTGGTCACATGGGCTTCTCAGGGGACTTCCAGTCATTTGTCAAAGACTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000002948-Map2k7:NM_001042557:9
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact





	

	
ORF disruption upon sequence inclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.111 A=NA C2=0.000
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0006920=Pkinase=FE(10.0=100)

							
A:
NA

							
C2:
PF0006920=Pkinase=FE(18.0=100)

						

					

				








    
Main Inclusion Isoform:
NA





     
                   









    
Main Skipping Isoform:
ENSMUSP00000050752





     
      









    
Other Inclusion Isoforms:
NA
          









    
Other Skipping Isoforms:
ENSMUSP00000003027, ENSMUSP00000054512, ENSMUSP00000106622, ENSMUSP00000106623, ENSMUSP00000106624, ENSMUSP00000106626, ENSMUSP00000106627, ENSMUSP00000117418
  



Associated events






Conservation


Human
(hg38)
chr19:7911331-7911414 
ALTERNATIVE
HsaINT0099014
(MAP2K7)
Human
(hg19)
chr19:7976216-7976320 
ALTERNATIVE
HsaINT0099014
(MAP2K7)
Rat
(rn6)
chr12:2549185-2549268 
LOW PSI
RnoINT0088922
(Map2k7)
Cow
(bosTau6)
chr7:17893364-17893486 
LOW PSI
BtaINT0089104
(MAP2K7)
Chicken
(galGal4)
chr14:4491382-4492638 
ALTERNATIVE
GgaINT0015627
(MAP2K3)
Chicken
(galGal3)
chr14:4510794-4512050 
LOW PSI
GgaINT0015627
(MAP2K3)
Zebrafish
(danRer10)
chr1:45161543-45162094 
LOW PSI
DreINT0092550
(map2k7)
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
TCCCACCAAGCCTGACTATGA

				
R: 
CTGAGAAGCCCATGTGACCAG

				
Band lengths: 
173-258

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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