MmuINT0103658 @ mm10
Intron Retention
Gene
ENSMUSG00000055775 | Myh8
Description
myosin, heavy polypeptide 8, skeletal muscle, perinatal [Source:MGI Symbol;Acc:MGI:1339712]
Coordinates
chr11:67292842-67294612:+
Coord C1 exon
chr11:67292842-67292978
Coord A exon
chr11:67292979-67294356
Coord C2 exon
chr11:67294357-67294612
Length
1378 bp
Sequences
Splice sites
5' ss Seq
AAGGTAACA
5' ss Score
8.92
3' ss Seq
ATCTTTCCCCCACATCCCAGAGA
3' ss Score
6.04
Exon sequences
Seq C1 exon
GTTTTCTTCAAAGCTGGTCTCCTGGGTCTCCTGGAAGAAATGAGAGATGAAAAGCTGGCCCAGATTATAACAAGAACCCAGGCGGTCTGTAGGGGATACCTAATGAGAGTGGAGTATCAGAAGATGCTGCTGAGAAG
Seq A exon
GTAACAGCTATGTATATTCTCGGGATCACAAAGTCAACCCCCACCCCACACCCTGGGGACTGTCCATCAGCTTTCCTCATTTACTGATCCTCTGATTCTGAGATTAGCATGAGCTTTCACCCCTACAACACATTAACCTTAGTGTCACCCTTCAGGCTAGACTTCCCAGAACTTCTCTCTTGACCTCTCGCCCCACCCCTACCCCCATCCATCTCACACACAGAAACATTCTGCCACAAGCACTTTTCATCAGCCCACATCTCTGTTTCCAGCTCTTCATGAAATTCCACATCAAATCCAGGCTGCTTTGCTTGGCTGTCAAGTTTCTATTCCCCCACCGTATCTTTCATATCTTTTTTCATATCTTTCATATCATGGCTTTTCCTAGCCATGCTAAACAGTTCACGTACCACTTATTTTCATGTCTGAACAGACCTGCCTAGTGACAAGTCCCTTTTCTTCTTTCCCAAATGAGCATCCTCCAACTTATCTACACAACATTTTTTTTTACATTGCTCTAGCCATGTACATATTTTACTAATTTTGTTCATCTGGATTTTTAAGGTTGGCATAATAAGAGTTTTGGTTGTCCCCCACCCCCAGTCCATCTGTATCACAGCTTGTGTCATGATATTTGCCTTCTCAGTGTTGGATTTCCTAGCAACCAAACCAATAGAAATAAAACTTGCCTGGTAACGAATTTAAAGATGCCTACATATTGCATAAGCCACCCAAACATTGCCAGCCCATTGCTATCATTTGAAGAGCTGTACCAAACTTACCATTTGAATTTTTTTATTTAAATAAAACAACTTTGGGGCTTACATGATGCTAGAAATGTCTCCACATGTTAATGAACTTATTGTGCTGGCAATACTAAGCCACTCAGAGAATTTAGAAAAGCCAGTATAGTACTTGAAGACAGCAACAAGAGAAGAGTAGCTAATTCGGTGGTGATTAATACAGGAGCAGTTTGAAGGAAAGAGTAGCTGGGCTGAGGTGAGGTGAACTCTTCAGCCTATGGCTTCTCACCTCGAAGCTCACAGTTGAGTAGCAGCGGCAGGATAGCACAGTCTGATTACAATGGTGGTGGCCTCCCATACACTTCTGCCACGTGTGTCTGCTCTTTCTACGGCAGGGGTTGCTCAGATGGTATAATCTGTACCTTCTACACTCAGCCCACCCTTGCCATCTTGGCAAGGAACTGAGAACACTCAATTGTTTCATATAGCAGAGTAGCAATACTGAGGACAATAGGCTCTAACTCCGATTGTCAGGATGAACACGCTACAGTACAGGAAAACCATGTTGCCTGCAATAAACGTCAATCACACTGCTGCCAGTGAAATGATTACCCCATCTTTCCCCCACATCCCAG
Seq C2 exon
AGAATCCATCTTCTGTATCCAGTACAACGTCCGCGCCTTCATGAACGTCAAGCACTGGCCCTGGATGAAACTCTTCTTCAAGATCAAACCCCTGCTGAAGAGCGCAGAGACCGAGAAGGAGATGGCCACCATGAAGGAGGAGTTCCAGAAAACCAAGGATGAGCTGGCCAAGTCGGAGGCAAAGCGGAAGGAGCTGGAGGAGAAGATGGTCACTCTTCTCAAAGAGAAAAATGACCTGCAACTCCAGGTTCAATCT
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000055775:ENSMUST00000019625:21
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.140
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=PD(0.4=6.5),PF0061222=IQ=WD(100=45.7)
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTTCAAAGCTGGTCTCCTGGG
R:
ACCATCTTCTCCTCCAGCTCC
Band lengths:
342-1720
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types