MmuINT0104459 @ mm10
Intron Retention
Gene
ENSMUSG00000025885 | Myo5b
Description
myosin VB [Source:MGI Symbol;Acc:MGI:106598]
Coordinates
chr18:74759027-74761024:+
Coord C1 exon
chr18:74759027-74759312
Coord A exon
chr18:74759313-74760849
Coord C2 exon
chr18:74760850-74761024
Length
1537 bp
Sequences
Splice sites
5' ss Seq
CAGGTGAGA
5' ss Score
9.22
3' ss Seq
TTCCTTTTGCCTTGGCTAAGGTA
3' ss Score
4.87
Exon sequences
Seq C1 exon
TTTCTGCCATGTTGGAAAATGAGAGTATCCAGGGGCTGTCTGGTGTGAGACCAACTGGTTACCGGAAGCGCTCCTCCAGCATGGTGGATGGAGAGAATTCGTATTGCCTGGAGGCCATCGTCCGCCAGATGAATTCTTTCCATACAGTCCTGTGTGACCAGGGTCTGGACCCCGAGATTATCCTGCAGGTGTTCAAACAGCTCTTCTACATGATCAACGCTGTGACTCTTAACAACCTACTCCTGCGGAAAGACGCCTGCTCCTGGAGCACAGGCATGCAACTCAG
Seq A exon
GTGAGAGGGTCACTCAGGCCAGCACACAGAGACCTGGAGTGGTCATCTGTTAACCTGCTCTGAGCTCTCGGTGTGTGTGCACTCTTCACTGCCTGTCAGAGTTCTCTGGATTCTTGAATGAAAACATGCACACACACACACACACACACACACATGTAACCTTTATATTTTGATATGTCTTAACTGTTCAATGGCTGGGCCACTCCTGAATCTCCGTGTGGCTAACACGCTCCCCTCTGATATTCCTGAGTTAATATTTACTGAATTTGTATCTCACCTCTGTTACTCCAGACCCAATCGGGGGAGTGGCCTCTGCGGCCATTTTTCCTGATTCTTACAAGTCAACGGGCTCCTGCAGCTCTTAAGTCTGATCTCTCAAACTCCCATCCTGGCAATTTTGCTCCTCCTCCTTCCCTTGACCTGGAAATCTGAAAGTCCCGCCTTCATCTCCCTGCCCAGCCATTGTTAGCAACTTTATTGACCAATCAAAAACCAATTGGGGACAGGGCCCCCTCAGCATCTGGACACTAGATCCCCATGTGATTTGGGGAGCCAAATTAAGGAGAAGCATTAGAACCACTCCCCAACAGAGACCAAGAGCCCCAACACAGGCTGGGGTCAGAACACAGGACAGCTGCCTTCAAGGTGAAATCTACACCTAACTGGTAGCCCTTCCCACAACTGTATGACTCAAATCCTCCACTTCCGTATTAATTGGTGAAGTTCACTCAGCAATGAAGCTCAAACTAGCTTAAGAGATGCTCGCCAGATACCTTTTGATTAAGACTAATGCAATGTGGCCAGGCCAGCGAGAAAGATGGATGAACTGGGAGTTATCAAGGCTGTCAAGGAAATATGAATGGTTGTAAGTTAAATACAAATTATAATTCAGAATGTTTAATGCTTCCCTCTGTTAATCTATATAGACACATTAGGTATTCCTTCTTTGCTTAGTTTTAAGTATGCATTCTATTATTCACTCTTCACTCAATATATGGCTACTGAATGCTAGGCACTTTGCCAAGTTTCATGTATGCATGTAAGTGTAGTGGTGCATACACGTGTAGGTGAACATGTGGAGGTTAGAGGACAGCTCAAACATGCACCTTTAAACAACAAACAAACAAACAACAAGCTCTCATTGGCCTGGAACTTATCACAGTTGTGTAGGCTGGCTGGTCAGCAAAACCTGTCTCCCCTCCTCGGCCCAGCACCTAGGCTACAACTGTGTGCCACCTCGCCCATTAGTGGTTTTGTTGTTTGGTGTGTGCGTGTGTGTGTGTGTGTGTGCATGTTTATGTATGAGTCTTGGGATCAACCTCAGGCCCTCTTGCTTGCAAGGCAAACATTTTACAAACTGAGCTATCTCCCTTACCCCGCTTTGCAAGCTTTGAACCACATGAAATAACTGAAGTGAGGGCATTGAGGTGAGGGCACTGTCACCTGCACAGCACAGCAGGCGCTCCCTCCCCACACCCCTCCCCATTTCCACCCCGAGAATTAAGGCTTAACTCGGCTTCCTTTTGCCTTGGCTAAG
Seq C2 exon
GTACAACATAAGTCAACTGGAAGAGTGGCTTCGGGGCAAAAACCTTCACCAAAGTGGAGCAGTTCAGACCATGGAGCCCCTCATCCAGGCAGCCCAGCTCCTCCAGCTGAAGAAGAAAACCCACGAGGATGCTGAGGCCATCTGCTCTCTGTGCACCTCCCTCAGCACCCAGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000025885:ENSMUST00000074157:36
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.010 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0184314=DIL=PU(31.1=34.4)
A:
NA
C2:
PF0184314=DIL=FE(54.7=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGAGTATCCAGGGGCTGTCTG
R:
GCTCCATGGTCTGAACTGCTC
Band lengths:
342-1879
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types