Special

MmuINT0127479 @ mm10

Intron Retention

Gene
ENSMUSG00000070371 | Prss36
Description
protease, serine 36 [Source:MGI Symbol;Acc:MGI:1924863]
Coordinates
chr7:127936325-127936838:-
Coord C1 exon
chr7:127936589-127936838
Coord A exon
chr7:127936455-127936588
Coord C2 exon
chr7:127936325-127936454
Length
134 bp
Sequences
Splice sites
5' ss Seq
CAGGTGAAC
5' ss Score
5.56
3' ss Seq
CAGGCCTCTCATCTCTTAAGAGT
3' ss Score
4.17
Exon sequences
Seq C1 exon
GGTGACTCTGGGGGACCGCTGGTCTGTGAGGACGGAGGCCGATGGTTCCTGGCCGGAATCACCAGCTTTGGCTTTGGCTGTGGACGGAGGAACCGCCCTGGGGTCTTCACTGCAGTGGCTCCCTATGAGTCGTGGATTCGGGAACACGTGATGGGTTCAGAACCTGGGCCTGTCTTTCCCAGCCAGCTCCAGAAGCCACAGTCAGGACCCTGGGAGCCCAGAGAAGAAAACTGTACCTTTGCTCAACCAG
Seq A exon
GTGAACGGCAGATGCACTGACACTGAGCTGAGCTGAGCTGGGCTGGGCTGGGCTGGGCAGATACGAATTGGGGTGGAGGCTGCAGGGTTAAGGATTTCTGCAGACTCCCTGACCCAGGCCTCTCATCTCTTAAG
Seq C2 exon
AGTGCGGGAAGGCTCCGAGGCCGGGGACCTGGCCCTGGGAGGCCCAAGTGACCGTACCAGGCTCCACACCCTGCTATGGAGCTCTGGTGTCTGACAGATGGGTCTTGGCACCCGCCAGCTGCTTTCTGGA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000070371:ENSMUST00000118755:7
Average complexity
IR
Mappability confidence:
NA
Protein Impact





	

	
ORF disruption upon sequence inclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.333 A=NA C2=0.170
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0008921=Trypsin=PD(18.8=53.6),PF0008921=Trypsin=PU(0.1=0.0)

							
A:
NA

							
C2:
PF0008921=Trypsin=PU(18.5=97.7)

						

					

				








    
Main Inclusion Isoform:
NA





     
                   









    
Main Skipping Isoform:
ENSMUSP00000091565





     
      









    
Other Inclusion Isoforms:
NA
          









    
Other Skipping Isoforms:
ENSMUSP00000112659
  



Associated events







Other assemblies


mm9




Conservation


Human
(hg38)
chr16:31143472-31143587 
LOW PSI
HsaINT1028217
(PRSS36)
Human
(hg19)
chr16:31154793-31154908 
HsaINT0132991
(PRSS36)
Rat
(rn6)
chr1:199383218-199383324 
ALTERNATIVE
RnoINT0118737
(Prss36)
Cow
(bosTau6)
chr25:27495862-27495970 
BtaINT0118748
(PRSS36)
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
chr3:39404382-39404473 
LOW PSI
DreINT0179697
(zgc:165423)
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
GGCTCCCTATGAGTCGTGGAT

				
R: 
GTGCCAAGACCCATCTGTCAG

				
Band lengths: 
245-379

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Ribosome-engaged transcriptomes of neuronal types
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types





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