MmuINT0130108 @ mm10
Intron Retention
Gene
ENSMUSG00000032604 | Qars
Description
glutaminyl-tRNA synthetase [Source:MGI Symbol;Acc:MGI:1915851]
Coordinates
chr9:108511532-108512582:+
Coord C1 exon
chr9:108511532-108511618
Coord A exon
chr9:108511619-108512482
Coord C2 exon
chr9:108512483-108512582
Length
864 bp
Sequences
Splice sites
5' ss Seq
AAGGTAAGC
5' ss Score
10.22
3' ss Seq
TGGTCCTCCAACTCTCTTAGGCC
3' ss Score
6.55
Exon sequences
Seq C1 exon
GTACGTACGCGGTTCCCTCCAGAGCCCAATGGAATCCTGCATATTGGACACGCCAAAGCCATCAATTTCAACTTTGGTTATGCCAAG
Seq A exon
GTAAGCATTTGTCTAGGTCCTGGCAAGTAATATGATCACTGTCTACTCCCATGACTGCATTCCAGAAAGGTATATTTCTGTCTTGCCTTTTGTAGCTGATGACAGAGGCCCTAGAGAAAAACTCCATGTAACAGCTATTGTCAGGAAATACTGGGTTAATGGGGAAAATTAAAATGGTTCTTTAGATAGGGTTTCACTGTAGAGTCCTGATGGCCTTGAATTGTGGCGATCACATGCCTCTGCTTCCCAGGTACTGGGATTAAAGGGGTGCACCGCTATGCCCCATGGAAGATGCATTTCTTCAATTCCTTATATTAACTTTTCAATTTATTTTTTGGCAGTCATCAATAGAAGACATTAATTAAAAAAATATTCCTAGGTGGCTGAAAAGATGGCTCATTGGTTAAGACCAGTTGTTGGCCTTGCAGAGAACTTGGTTCAGTTCCCATCACCCACATGGTGGCTTATTCAGTTTTCGGGGATGTTTTCTTCTGAAATCATCTTTTGCCCTCTGAGGGCACTGGGCACACACATGGTGAAAGCATATGCATGCAGGCAAAACACTCAAACACATAAATCTAAAGGGAAAAAAATGATGTGTGTGTGTGCACGAGCGCAAGCACTCATGCATACTCATGCTATTGAACATTCATGGATGTCAAAGACAGACTTTGGTGGAGTTGGTTCTTTTCATCATAGACTTTGAGGATCCAACTGAGGTCATTCAGCTTGCTAGGCAACTCCTCTTATCTGCTGAACTATCTCATTGGCTGCCTTTTACATTTGATAAGCTTGGACTTTGAGAATGTAAGGCTAATGGTATGGACTCTAGGGCTGATGGGTGTGGTCCTCCAACTCTCTTAG
Seq C2 exon
GCCAACAATGGTATCTGTTTTCTGCGCTTTGATGACACCAACCCTGAGAAAGAAGAAGCAAAATTCTTCACTGCTATTTATGACATGGTGACCTGGCTGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000032604:ENSMUST00000006838:10
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.051 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0455710=tRNA_synt_1c_R2=PD(10.0=26.7)
A:
NA
C2:
PF0074916=tRNA-synt_1c=FE(11.0=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TACGCGGTTCCCTCCAGAG
R:
CCAGCCAGGTCACCATGTCAT
Band lengths:
182-1046
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types