RnoEX0078739 @ rn6
Exon Skipping
Gene
ENSRNOG00000013123 | Scube2
Description
signal peptide, CUB domain and EGF like domain containing 2 [Source:RGD Symbol;Acc:1563998]
Coordinates
chr1:174438657-174454769:-
Coord C1 exon
chr1:174454563-174454769
Coord A exon
chr1:174440102-174440272
Coord C2 exon
chr1:174438657-174438824
Length
171 bp
Sequences
Splice sites
3' ss Seq
CACTGACCCGCTTTGGCTAGTCA
3' ss Score
1.81
5' ss Seq
GAGGTAAGG
5' ss Score
10.28
Exon sequences
Seq C1 exon
CTTCCTGCAATCTGAGCTGCGTGGTGAAGCGAACAGAGAAGAGGCTGCGGAAAGCTGTGCGCACACTCAAGAAGGCTGCCCACAGGGAACAGTTTCACCTGCAGCTCTCAGGCACGGACCTCGATGTGGCTAAAAAGTTTCCCCGAGTGTCGGGACAGCAGGAGGAGACCTGTGGAGTGGGCCAGGGTCGTGAAGAAAGCCAGTGTG
Seq A exon
TCAGTTGCAGGGCTGGGACGTATTATGATGGATCACAAGAACGCTGCATTTTATGTCCAAACTGAACCTTCCAAAATGAGGAAGGACAAGTCACTTGTGAACCATGCCCAAGACCAGAAAATCTTGGGTCCCTAAAAGTCTCAGAAGCCTGGAATGTATCTGACTGTGGAG
Seq C2 exon
GCCTTTGTCAGCCGGGTGAATATTCAGCAGATGGATTTGCCCCTTGCCAGCCCTGTGCCCTGGGCACCTTTCAGCCTGATGTGGGCCGTACATCTTGCCTCCCGTGTGGAGGAGGTCTTCCTACTAAGCATCTGGGAGCCACTTCCTTCCATGATTGTGAAACCAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000013123_CASSETTE1
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.057 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF076998=GCC2_GCC3=PU(10.2=8.6)
A:
NO
C2:
PF076998=GCC2_GCC3=PD(88.1=91.2)
Main Inclusion Isoform:
ENSRNOT00000052377fB13020
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AATCTGAGCTGCGTGGTGAAG
R:
AAGGGGCAAATCCATCTGCTG
Band lengths:
244-415
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]