RnoEX6016719 @ rn6
Exon Skipping
Gene
ENSRNOG00000027596 | Mbd5
Description
methyl-CpG binding domain protein 5 [Source:RGD Symbol;Acc:1562961]
Coordinates
chr3:33465220-33473531:+
Coord C1 exon
chr3:33465220-33465546
Coord A exon
chr3:33467689-33467909
Coord C2 exon
chr3:33472323-33473531
Length
221 bp
Sequences
Splice sites
3' ss Seq
TTTGTTTTTCAAAACTGTAGGCG
3' ss Score
6.84
5' ss Seq
CAGGTACTT
5' ss Score
8.17
Exon sequences
Seq C1 exon
GTGGTTCAAGCCTGCCGTCCTCCATAGCTATAGCTGGCAGCAGCCACCCTGCCATCACAAAGACAACATCTGTTCTCCAAGATGGTGTCATAGTCACCACCGCAGCCGGAAACCCACTGCAGAGTCAGCTGCCCATTGGGAGTGACTTTCCTTTTGTTGGCCAAGAGCATGCCCTTCATTTTCCATCCAACAGCACTTCAAACAACCATCTTCCACACCCCTTGAACCCCAGCCTCCTCAGTTCTCTACCTATCTCTTTGCCAGTGAATCAACAGCATCTCCTAAACCAGAATCTATTAAATATCCTCCAGCCTTCAGCAGGAGAAG
Seq A exon
GCGATATGTCATCCATAAACAGTTCTCTGAATAACCATCAACTGACTCACCTACAGTCACTGCTAAACAGCAGTCAGATGTTTCCTCCGAGTCAGCAGCAGCCGCCGCCGCAGCCGCACCTTCTCCAGGGACAGCAGAATCTCCAGGCCTTCCAAGGACAACCCACAGTCCTTTGCCCAGCTAACAATAACCCCATGGCATGTCTGTTTCAGAACTTCCAG
Seq C2 exon
GTGAGGATGCAAGGAGATGCAGCTCTTCTAAATAAGAGAATAAACAGTCAGCCGGGGCTCACAGCACTTCCTGAGAACCCAAACCTTGCACTTCCACATTTCCAAGATACACCTTGTGAGCTGCAGCCAAGGATTGACCCGTCTCTTGGTCAGCCCATGAAGGACGGCCTCGTTATGGGTGGCCAAGGCGATGCCTCCGTAGACGCCATTTACAAAGCAGTTGTGGATGCAGCCAGCAAAGGAATGCAGGTTGTCATCACCACTGCTGTGAACAGCACCACTCAAATCAGCCCTATCCCAGCCCTGAGCGCCATGAGTGCCTTCACTGCCTCCATTGGTGACCCACTAAATCTGTCCAGTGCTGTGAGCGCCGTCATCCATGGGCGGAACATGGGAGGAGCGGATCACGACAGCAGGCTGAGGAATGCGAGAGGGGCGCGGCTACCCAAGAACATAGACCATGGGAAAAACTCTGGTGAAGGAGACGGGTTTGAGTGTTACAAGTCAGCGAGCTGCCACACATCCAGAAAACAATGGGATGGAGAGCAAAGTCCCAGAGGGGAGCGTAACAGGTGGAAATATGAGGAGTTTTTAGATCATCCAGGCCATATCCACAATAGTCCTTGTCATGAAAGGCCCAACAATGTCTCCACACTGCCATTTCTAGCTGGGGAACAGCACCCAATACTGTTACCACCAAGAAACTGTCAAGGTGATAAAATTCTGGAGGAAAATTTCAGGTATAATAACTACAAAAGAACTATGATGAGTTTTAAAGAGAGACTAGAGAGTACTGTGGAGAGATGTACACACATTAATGGAAACAGACCTCGTCAGAGTCGGGGCTTTGGAGAGCTGCTAGGCACTGCAAAACAAGACCTAGTCCTAGAGGGGCAGTCTCCAGGCTCTTCGAATAGTTTGGAAAGTTCTCTGGTCAAAGACTACATCCATTACAATGGAGACTTTAATGCCAAAAGCATTAATGGGTGTGTGCCTAGTCCCTCCGATGCTAAAAGCATTAGTAGTGAAGACGACCTAAGGAATCCAGACTCCCCCTCTTCTCATGAGTTGATACACTATAGACCAAGGACGTTCAACGTTGGCGACTTGGTCTGGGGCCAAATCAAAGGACTGACTTCCTGGCCTGGAAAATTCATAAGAGAAGACGACGTTCACAATTCATGCCAACAAAGCCCCGAGGAAGGGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000027596-'4-22,'4-13,13-22=AN
Average complexity
A_C3
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.344 A=0.598 C2=0.514
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
PF0085512=PWWP=PU(46.3=9.4)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCCCTCGCTGAGCACAGT
R:
TTTGTCCAGTTCGCTCATGGC
Band lengths:
156-377
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]