RnoEX6016868 @ rn6
Exon Skipping
Gene
ENSRNOG00000007666 | Cacnb4
Description
calcium voltage-gated channel auxiliary subunit beta 4 [Source:RGD Symbol;Acc:68385]
Coordinates
chr3:37989998-37997559:-
Coord C1 exon
chr3:37997437-37997559
Coord A exon
chr3:37992459-37992589
Coord C2 exon
chr3:37989998-37990074
Length
131 bp
Sequences
Splice sites
3' ss Seq
CTTTCCTCTCTGCGTTCCAGAAA
3' ss Score
10.06
5' ss Seq
CGGGTAACA
5' ss Score
5.3
Exon sequences
Seq C1 exon
TCCAAACCTGTAGCTTTTGCGGTGAAAACGAATGTGAGCTACTGTGGTGCCCTGGATGAGGATGTGCCCGTTCCCAGCACAGCCATCTCCTTTGACGCCAAGGACTTTCTTCACATTAAAGAG
Seq A exon
AAATATAACAATGATTGGTGGATAGGAAGGCTGGTGAAGGAGGGCTGTGAGATCGGCTTCATCCCAAGTCCACTGCGTTTGGAGAACATACGGATTCAACAGGAACAGAAAAGAGGCCGTTTTCATGGCGG
Seq C2 exon
GAAATCGAGTGGAAACTCTTCCTCCAGTCTGGGAGAAATGGTGTCAGGAACATTTCGAGCAACTCCCACAACAACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000007666-'7-6,'7-5,9-6=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.227 C2=0.963
Domain overlap (PFAM):
C1:
PF120523=VGCC_beta4Aa_N=PD(2.4=2.4),PF0001823=SH3_1=PU(44.2=46.3)
A:
PF0001823=SH3_1=PD(51.2=50.0)
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Mouse
(mm9)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCTTTTGCGGTGAAAACGAATG
R:
GTTGTTGTGGGAGTTGCTCGA
Band lengths:
186-317
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]