RnoEX6024928 @ rn6
Exon Skipping
Gene
ENSRNOG00000020205 | Agrn
Description
agrin [Source:RGD Symbol;Acc:2067]
Coordinates
chr5:173591574-173594162:-
Coord C1 exon
chr5:173594075-173594162
Coord A exon
chr5:173591956-173592180
Coord C2 exon
chr5:173591574-173591677
Length
225 bp
Sequences
Splice sites
3' ss Seq
CCCACCCTTTTGCTTTTCAGTGA
3' ss Score
9.66
5' ss Seq
CAGGTGAGT
5' ss Score
10.67
Exon sequences
Seq C1 exon
GGCTAGTTGAGAAGTCAGTGGGGGACCTAGAAACACTGGCCTTTGATGGGCGGACCTACATCGAATACCTCAATGCTGTGATTGAGAG
Seq A exon
TGAGAAGGCACTGCAGAGCAACCACTTTGAACTGAGCTTACGCACTGAGGCCACTCAGGGGCTGGTGCTGTGGATTGGCAAGGCTGCAGAACGTGCAGATTACATGGCCCTGGCCATCGTGGACGGGCACCTGCAACTGAGCTATGACCTAGGTTCCCAGCCAGTTGTGCTGCGCTCCACTGTGAAGGTCAACACCAACCGCTGGCTTCGAATCAGGGCTCACAG
Seq C2 exon
GGAGCACAGGGAAGGTTCCCTTCAGGTGGGCAATGAAGCCCCTGTGACTGGATCTTCCCCATTGGGTGCCACGCAACTGGACACAGATGGAGCCCTGTGGCTTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000020205-'58-63,'58-61,61-63=AN
Average complexity
A_C1
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.458
Domain overlap (PFAM):
C1:
NO
A:
PF0005418=Laminin_G_1=PU(47.0=81.6)
C2:
PF0005418=Laminin_G_1=FE(26.5=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGCTAGTTGAGAAGTCAGTGGG
R:
CCACAGGGCTCCATCTGTGTC
Band lengths:
188-413
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]