BtaEX0021020 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000021024 | MACF1
Description
microtubule-actin crosslinking factor 1 [Source:HGNC Symbol;Acc:HGNC:13664]
Coordinates
chr3:107373814-107378553:-
Coord C1 exon
chr3:107378464-107378553
Coord A exon
chr3:107375946-107376041
Coord C2 exon
chr3:107373814-107373891
Length
96 bp
Sequences
Splice sites
3' ss Seq
GCCTTGGTTGGTGGCCAAAGGAG
3' ss Score
0.53
5' ss Seq
GTCGTAGGT
5' ss Score
4.05
Exon sequences
Seq C1 exon
GTCCGCAAGCACATAAATGACCTTTATGAAGACCTGCGGGATGGCCATAACCTGATCTCTCTGCTGGAGGTCCTCTCGGGCATCAAACTG
Seq A exon
GAGCCGGCAGGGCTGAAGACCCTCCGCCTGGTGAGCATGCCCTCCTGGCGCCGTGCAAACAGCGAGGAGCAGGAGGAGGAAGATGATGATGATGTC
Seq C2 exon
CCCCGGGAGAAGGGCAGGATGCGTTTTCACAGGCTGCAGAACGTGCAGATTGCCCTGGACTTCCTAAAGCAGCGACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000021024_CASSETTE7
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.438 C2=0.017
Domain overlap (PFAM):
C1:
PF0030726=CH=FE(28.7=100)
A:
PF0030726=CH=FE(23.3=100)
C2:
PF0030726=CH=FE(24.8=100)
Main Inclusion Isoform:
ENSBTAT00000033270fB4010
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTCCGCAAGCACATAAATGACC
R:
TGTCGCTGCTTTAGGAAGTCC
Band lengths:
167-263
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development