BtaEX0034192 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000032544 | SPAG1
Description
sperm associated antigen 1 [Source:HGNC Symbol;Acc:HGNC:11212]
Coordinates
chr14:66321916-66334699:-
Coord C1 exon
chr14:66334533-66334699
Coord A exon
chr14:66330873-66331005
Coord C2 exon
chr14:66321916-66322080
Length
133 bp
Sequences
Splice sites
3' ss Seq
TTTTTGTGTGTGTTCCATAGATG
3' ss Score
11.24
5' ss Seq
CAGGCAAGT
5' ss Score
3.1
Exon sequences
Seq C1 exon
GATAACAAAGATTTTGATCGCTCTGGATGGCCCAAACTGGCGGGAGAAGCTGCCACCCATACCTGCTGTGCCCACCTCCACACAAGTCCGGGCTTGGCGGCCTGCAGCAGAGACGCCCCCAGACCAAGTGGGAGATTCCTGTTGCCATCCCCAGCCGGGCATGCCAG
Seq A exon
ATGAAAACGTATTTAAAACCCTTAAAGAAGAAGGAAATCAATGTGTAAAAGACAAAAACTATAAAGATGCCCTTAGTAAATACAGTGAATGCTTGAAGATTAACAACAAGGAATGTGCCATTTATACAAACAG
Seq C2 exon
AATTATCAGAAAAGTTTAAACGATCTCAATAAAGTTCTCCTGCTAGACCCAAGTATTGTTGAGGCAAAAATGGAACTGGAAGAGGTAACCAGAATCCTTAATATTAAGGATAATACAGCATCATTCAACAAAGAAAAGGAGAGAAGGAAAATTGAGATTCAAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000032544_MULTIEX1-1/2=C1-C2
Average complexity
C2
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.386 A=0.067 C2=0.127
Domain overlap (PFAM):
C1:
NO
A:
PF134141=TPR_11=PU(60.9=93.3)
C2:
PF0051523=TPR_1=PD(52.9=32.7)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTTTGATCGCTCTGGATGGCC
R:
TCTGGTTACCTCTTCCAGTTCCA
Band lengths:
249-382
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development