GgaEX0023632 @ galGal4
Exon Skipping
Gene
ENSGALG00000016037 | SPAG1
Description
sperm associated antigen 1 [Source:HGNC Symbol;Acc:HGNC:11212]
Coordinates
chr2:128200043-128206313:+
Coord C1 exon
chr2:128200043-128200218
Coord A exon
chr2:128202726-128202867
Coord C2 exon
chr2:128206149-128206313
Length
142 bp
Sequences
Splice sites
3' ss Seq
GTGATCCTTTCGTTATGTAGGTG
3' ss Score
8.75
5' ss Seq
CAGGTAACG
5' ss Score
10.52
Exon sequences
Seq C1 exon
AATAACGAAGACTTTAATTGATCAAGATGGTTCTAGTTGGAGGGAAAAACTGCCACCGATTCCAGTTGTCCCTATTGCTGCTCAGCTAAACAGGTGGGATGGAGGAAACTTTACTTCAGAGGTTAAGCCAAAAAGTCCCACAGATATCAACAAAGAAGAACAGTTGCAGATGAATC
Seq A exon
GTGAGAAAGCTGAGGAAAAGTTCAGGACATTAAAAAATGAAGGGAATGATTTTGTGAAGAAGGGTAAATATGATGAAGCTGTAAATAAATACAGTGAATGCTTGAAGTTAAATACCAAGGACTGCACAATATACACTAACAG
Seq C2 exon
AGCTATCAAGCCAGTGTTGATGATCTCAAGAAAGTACTTCTAATAGATCCAAATGTTCTTGAAGCAAAAAAGGAACTGGAGGAGATAACCCAACTGCTCAGCCTTGGCGGCGTTGCTGTTGCTGACTGTCAACAAAAGCAAAGGAAGAAAATAACCATACAAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000016037_MULTIEX1-4/5=3-C2
Average complexity
C2
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.350 A=0.125 C2=0.018
Domain overlap (PFAM):
C1:
NO
A:
PF134141=TPR_11=PU(60.0=87.5)
C2:
PF0051523=TPR_1=PD(52.9=32.7)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTGGAGGGAAAAACTGCCACC
R:
CAAGGCTGAGCAGTTGGGTTA
Band lengths:
246-388
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]