DreEX0074616 @ danRer10
Exon Skipping
Gene
ENSDARG00000004017 | spag1a
Description
sperm associated antigen 1a [Source:ZFIN;Acc:ZDB-GENE-030131-9443]
Coordinates
chr19:12076703-12087349:+
Coord C1 exon
chr19:12076703-12076857
Coord A exon
chr19:12083987-12084128
Coord C2 exon
chr19:12087223-12087349
Length
142 bp
Sequences
Splice sites
3' ss Seq
TGTATCTACTTTCTCCACAGCCA
3' ss Score
9.54
5' ss Seq
CAGGTATAC
5' ss Score
7.48
Exon sequences
Seq C1 exon
AATCACTAAAATGCTGATTGAGCAGGACGGTCCTGACTGGCGAGAGAAGCTTCCAGAGATTCCAGCGGTTCCTCTTTCTGCTCAGCAGCACCGCAAAGAAGAGCCCAGCGCAGAGCTCCTGCAGGCCCGAGCAGAAAGAGCAGAACAGGAGAAAG
Seq A exon
CCAGAAAAGCAGAGGCTCGATTCACAATACTGAAACAAGAAGGAAATGAGCTGGTGAAGAACAGCCAGTTTCAAGGTGCCTCGGAAAAATACAGCGAGTGTCTGGCCATCAAACCCAACGAATGTGCCATTTACACCAACAG
Seq C2 exon
AGCGCTGTGCTTTCTCAAGCTGGAACGTTTCGCAGAAGCCAAACAGGACTGTGATTCTGCACTTCAGATGGAGCCAAAGAACAAGAAAGCGTTTTATAGACGAGCACTGGCACACAAAGGCTTGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000004017-'3-4,'3-3,4-4
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.419 A=0.052 C2=0.047
Domain overlap (PFAM):
C1:
NO
A:
PF134141=TPR_11=PU(62.9=91.7)
C2:
PF134141=TPR_11=PD(35.7=58.1),PF0051523=TPR_1=PU(39.4=30.2)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGACTGGCGAGAGAAGCTTC
R:
TTCAAGCCTTTGTGTGCCAGT
Band lengths:
249-391
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]