BtaEX6009005 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000020984 | RAPGEF4
Description
Rap guanine nucleotide exchange factor (GEF) 4 [Source:HGNC Symbol;Acc:HGNC:16626]
Coordinates
chr2:23703207-23710873:-
Coord C1 exon
chr2:23710727-23710873
Coord A exon
chr2:23708856-23708971
Coord C2 exon
chr2:23703207-23703295
Length
116 bp
Sequences
Splice sites
3' ss Seq
GGTGTAATTTGTATTTGCAGGAC
3' ss Score
7.32
5' ss Seq
AAAGTAGGT
5' ss Score
6.84
Exon sequences
Seq C1 exon
GGTGTGGTCTGTACCCTGCATGAGGGCGATGACTTTGGCAAGTTAGCCCTAGTGAATGATGCCCCAAGAGCTGCCTCCATCGTCTTGAGAGAAGATAACTGCCATTTCTTAAGAGTAGACAAGGAGGATTTCAACCGGATCCTGAGG
Seq A exon
GACGTTGAGGCGAATACAGTCAGACTTAAAGAACATGACCAAGATGTCTTGGTGCTGGAGAAGGTCCCAGCAGGGAACAGGGCTTCTAATCAAGGAAACTCACAGCCTCAGCAAAA
Seq C2 exon
GTATACTGTGATGTCAGGAACACCTGAAAAAATTTTAGAGCATTTTCTAGAAACGATACGCCTTGAGCCAGCTTTAAATGAAGCAACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000020984-'29-26,'29-25,30-26=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.359 C2=0.161
Domain overlap (PFAM):
C1:
PF0002724=cNMP_binding=FE(55.2=100)
A:
PF0002724=cNMP_binding=PD(2.3=5.1)
C2:
PF0061815=RasGEF_N=PU(26.2=90.3)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Primers PCR
Suggestions for RT-PCR validation
F:
GTGTGGTCTGTACCCTGCATG
R:
CATTTAAAGCTGGCTCAAGGCG
Band lengths:
226-342
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]