Special

MmuEX6102150 @ mm10

Exon Skipping

Gene
Description
Rap guanine nucleotide exchange factor (GEF) 4 [Source:MGI Symbol;Acc:MGI:1917723]
Coordinates
chr2:72198759-72205743:+
Coord C1 exon
chr2:72198759-72198905
Coord A exon
chr2:72201048-72201163
Coord C2 exon
chr2:72205655-72205743
Length
116 bp
Sequences
Splice sites
3' ss Seq
GGTATAATTTGTGTTTGCAGGAC
3' ss Score
7.17
5' ss Seq
AAAGTATGT
5' ss Score
6.93
Exon sequences
Seq C1 exon
GGTGTGGTCTGCACCCTGCACGAAGGAGATGACTTTGGCAAGTTAGCTCTAGTGAACGATGCTCCAAGAGCTGCCTCCATTGTTCTTCGGGAAGATAATTGTCACTTCCTAAGAGTCGACAAGGAAGACTTCAATCGGATTCTGAGG
Seq A exon
GACGTTGAGGCGAATACAGTCAGACTTAAAGAACATGACCAAGATGTCTTGGTACTGGAGAAGGTCCCAGCAGGGAACAGAGCTGCTAATCAAGGAAACTCACAGCCTCAGCAAAA
Seq C2 exon
GTATACTGTGATGTCAGGAACACCTGAAAAGATTTTAGAGCATTTTCTAGAAACAATACGCCTTGAGCCATCGTTGAATGAAGCAACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000049044-'61-35,'61-34,62-35
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact

ORF disruption upon sequence exclusion

No structure available
Features
Disorder rate (Iupred):
  C1=0.000 A=0.333 C2=0.161
Domain overlap (PFAM):

C1:
PF0002724=cNMP_binding=FE(55.2=100)
A:
PF0002724=cNMP_binding=PD(2.3=5.1)
C2:
PF0061815=RasGEF_N=PU(26.2=90.3)


Main Inclusion Isoform:


Main Skipping Isoform:
NA


Other Inclusion Isoforms:


Other Skipping Isoforms:
NA
Associated events
Other assemblies
Primers PCR
Suggestions for RT-PCR validation
F:
GTGTGGTCTGCACCCTGC
R:
TGTTGCTTCATTCAACGATGGC
Band lengths:
234-350
Functional annotations
There are 0 annotated functions for this event


GENOMIC CONTEXT[edit]

INCLUSION PATTERN[edit]


SPECIAL DATASETS

  • Ribosome-engaged transcriptomes of neuronal types
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types