BtaEX6018663 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000021024 | MACF1
Description
microtubule-actin crosslinking factor 1 [Source:HGNC Symbol;Acc:HGNC:13664]
Coordinates
chr3:107315500-107317673:-
Coord C1 exon
chr3:107317480-107317673
Coord A exon
chr3:107316303-107316465
Coord C2 exon
chr3:107315500-107315583
Length
163 bp
Sequences
Splice sites
3' ss Seq
GTTTCCCTTTGCTTCTGCAGCCA
3' ss Score
9.3
5' ss Seq
ACGGTGGGT
5' ss Score
8.27
Exon sequences
Seq C1 exon
CACTGGCTTAGTGATGTGAAGGACAAGAATTCAGTGTTTTCAGTCCTGGATGAGGAAATTGGCAAAGCCAAGATAGTGGCAGAGCAGCTGAGCCGTCTGACCCCAGAGCGAAACCTGGATTTGGAGCGCTGTCAGGAAAAAGGTTCCCAGCTACAAGACCGTTGGCACCGGGTCATTGCCCAGCTCGAGACCCG
Seq A exon
CCAATCTGAGCTAGAAAGTATCCAGGAAGTCCTGGGAGATTACCGAGCCTGCCATGGAACTCTCATCAAATGGATTGAGGAAACCACTGCCCAGCAGGAAATGATGAAGCCAGGCCAGGCTGAGGACAGCAGAGTACTCTCCGAGCAGCTCAGCCAGCAGACG
Seq C2 exon
GAGCTGTTTGCAGAAATTGAGAGAAATCAGACGAAACTGGACCAGTGTCAAAAATTTTCCCAGCAATACTCCACCATTGTAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000021024-'33-35,'33-34,34-35=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.015 A=0.273 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACTGGCTTAGTGATGTGAAGGAC
R:
TGACACTGGTCCAGTTTCGTC
Band lengths:
243-406
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]