RnoEX0051743 @ rn6
Exon Skipping
Gene
ENSRNOG00000016047 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:RGD Symbol;Acc:1306057]
Coordinates
chr5:141171789-141174241:-
Coord C1 exon
chr5:141174048-141174241
Coord A exon
chr5:141172676-141172834
Coord C2 exon
chr5:141171789-141171872
Length
159 bp
Sequences
Splice sites
3' ss Seq
CCCTTTGACCCTCCAGGCAGTCG
3' ss Score
3.07
5' ss Seq
ACGGTGAGT
5' ss Score
11.45
Exon sequences
Seq C1 exon
CACTGGCTTAGTGATGTGAAAGACAAGAATTCAGTATTCTCAGTCCTGGATGAGGAAATCTCTAAGGCCAAGGCAGTGGCAGAACAGCTCCATCACCGAGCAGCAGAGCCGAACTTGGATTTGGAGCGTTATCAGGAGAAAGGCTCGCAGCTTCAGGAGCGATGGCACCGTGTCATTGCCCAGCTGGAGACCCG
Seq A exon
TCGGAGGTAGAAAGTATCCAGGAAGTTCTGAGAGATTACCGAGCCTGCCATGGGACTCTCATCAAGTGGATTGAGGAAACCACTGCCCAGCAGGAAATGATGAAGCCGGGCCAGGCAGAGGATAGCAGAGTGCTGTCTGAGCAGCTTAGTCAGCAGACG
Seq C2 exon
GAACTGTTTGCTGAGATTGAGAGAAATCAGACAAAACTGGACCAATGTCAAAAACTTTCCCAGCAGTACTCCACTACTGTAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000016047-'51-50,'51-49,52-50
Average complexity
S*
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.154 A=0.273 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg38)
No conservation detected
Mouse
(mm10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACTGGCTTAGTGATGTGAAAGACA
R:
GCTGGGAAAGTTTTTGACATTGGT
Band lengths:
257-416
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]