DreEX6073698 @ danRer10
Exon Skipping
Gene
ENSDARG00000028533 | macf1a
Description
microtubule-actin crosslinking factor 1a [Source:ZFIN;Acc:ZDB-GENE-030131-3606]
Coordinates
chr19:35570286-35572721:-
Coord C1 exon
chr19:35572528-35572721
Coord A exon
chr19:35570450-35570609
Coord C2 exon
chr19:35570286-35570369
Length
160 bp
Sequences
Splice sites
3' ss Seq
CAAAACCTGATATCCTACAGTCA
3' ss Score
3.74
5' ss Seq
ACTGTGAGA
5' ss Score
2.78
Exon sequences
Seq C1 exon
CAATGGCACTCGGAGCTGCAGGAGCAGGATCACATATTTCAGAGTTTGAGTTTAGAGGTCCAGCAGGCTAGAGAGGTGGGCAACCAGCTCAGCCAGCTTCACCCGGACCGCAGCCCTGAATTGGACCGCTATCAGGAGAAAGCACACCAGCTTACAGACAGGTGGAGTGGAGTCCGCAGGCAGATGGAGACACG
Seq A exon
TCAAGCGGATCTGGAGATGCTGGGATCTGTTCTGCAGCAGTATAGAGAAGGCCACACTTCTCTCATACGCTGGATAGAGGAGACCACCGAAAAACAGGAGAACACACAGCCCGAACAAACTGACAGCAGAGCTTTGTCAGAACAGCTCGCACAGCAAACT
Seq C2 exon
GCATTGGTAGTTGAGATTGAACAGAACCAGGTCAAACTGGATGAGTGCCAGACCTGCTCTAAACAGTACAGCGCTGCCGTTAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000028533-'34-40,'34-38,35-40=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.385 A=0.426 C2=0.000
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=FE(56.1=100)
A:
PF0043516=Spectrin=PD(5.3=11.1)
C2:
NO
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAATGGCACTCGGAGCTGC
R:
AGGTCTGGCACTCATCCAGTT
Band lengths:
249-409
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]