BtaEX6018688 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000021024 | MACF1
Description
microtubule-actin crosslinking factor 1 [Source:HGNC Symbol;Acc:HGNC:13664]
Coordinates
chr3:107228118-107234053:-
Coord C1 exon
chr3:107233887-107234053
Coord A exon
chr3:107230114-107230273
Coord C2 exon
chr3:107228118-107228202
Length
160 bp
Sequences
Splice sites
3' ss Seq
TGTTCTATATATTCGTGTAGGCA
3' ss Score
6.18
5' ss Seq
AAGGTATGA
5' ss Score
9.11
Exon sequences
Seq C1 exon
TTGCTCCAGAGGCTACTAGATGATCGAAAGGCCACAGTAGACATGCTTCAAGCAGAAGGAGGCAGAATAGCACAGTCAGCTGAGCTGGCTGATAGAGAGAAGATCACTGGACAGTTGGAGAGTCTTGAAAGTAGATGGACTGGACTACTCAGCAAGGCAACAGCCAG
Seq A exon
GCAGAAACAGCTGGAAGATATCCTGGTTCTGGCCAAACAATTCCATGAGACAGCTGAGCCTATTTCTGACTTCTTATCTGTCACAGAGAAAAAGCTCGCCAACTCAGAACCTGTTGGCACCCAGACTGCTAAAATACAGCAGCAGATCATTCGGCACAAG
Seq C2 exon
GCTTTAAATGAAGAAATTGTTAATAGAAAGAAGAATGTAGATCAAGCTATTAAAAACGGTCAGGCTCTTCTGAAACAAACCACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000021024-'74-76,'74-73,75-76=AN
Average complexity
A_C2
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.011 A=0.109 C2=0.030
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=FE(51.4=100)
A:
PF0043516=Spectrin=PD(5.6=11.1),PF0043516=Spectrin=PU(41.1=81.5)
C2:
PF0043516=Spectrin=FE(26.2=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGCTCCAGAGGCTACTAGATGA
R:
GTTTCAGAAGAGCCTGACCGT
Band lengths:
242-402
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]