DreEX0044245 @ danRer10
Exon Skipping
Gene
ENSDARG00000028533 | macf1a
Description
microtubule-actin crosslinking factor 1a [Source:ZFIN;Acc:ZDB-GENE-030131-3606]
Coordinates
chr19:35495015-35496222:-
Coord C1 exon
chr19:35496056-35496222
Coord A exon
chr19:35495743-35495902
Coord C2 exon
chr19:35495015-35495341
Length
160 bp
Sequences
Splice sites
3' ss Seq
TTCTCATGTCACGTCCTCAGGCA
3' ss Score
8.41
5' ss Seq
AAGGTAGAG
5' ss Score
6.38
Exon sequences
Seq C1 exon
CTTCTGCAACGGTTGCTGGATGACCGCCGTGGGACTGTAGAGATGATCAGGGCCGAAGGAGAGCGTATCGCTGCTACAGCCGAAACACAAGACCGAGACAAAATCCAGAAACAGCTTCAGAGTTTAGGAGAAAGATGGACAAACCTGCTGGAAAAGGCCAGCGCCAG
Seq A exon
GCAGAAGCAGCTCGAGGAACTGCAGGTGCTGGCATTACAGTTCCATGAGGCCGTGGAGCCGCTCAGTGAGTGGCTGAGCGCAACAGAGAGACGCCTGAGCTCTGCTGAGCCAATGGGAACCCAGACATCCAAAATCACACAGCAGATTGTAAGGCACAAG
Seq C2 exon
GCTCTCCAAGAGGAAGTCTCCTCTCGAGAGAATAATGTAGACCGTCTTGAGAGTCTCAGCCAATCGCTCCATCCGCTCAGCTGCGCAGCCGATCAGGATTGGCTGGGCGAGCGTGTGGGTGCAGTGCGCAGCGGGCACACAGAGCTGCGTGATTGGTGCGTTCGGAGAGAGGCCATGCTGGAGCAGGCGCTGGCCAATGCACAGCTGTTTGGAGAGGAAGAGGTGGAGGTCCTGAACTGGCTAGCTGAGGTGGCCCAGAGACTGGCTGAGGTCTCAGTGCAGAGTTATCAGCCGGAGCACCTAGAGCAGCAGCACAAACACACACTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000028533-'73-77,'73-75,75-77
Average complexity
C1
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.321 A=0.333 C2=0.147
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=FE(51.4=100)
A:
PF0043516=Spectrin=PD(5.6=11.1),PF0043516=Spectrin=PU(41.1=81.5)
C2:
PF0043516=Spectrin=PD(57.0=56.0),PF0043516=Spectrin=PU(41.1=40.4)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAAACCTGCTGGAAAAGGCCA
R:
CCTCTCCAAACAGCTGTGCAT
Band lengths:
245-405
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]