Special

MmuEX0027463 @ mm9

Exon Skipping

Gene
ENSMUSG00000028649 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:MGI Symbol;Acc:MGI:108559]
Coordinates
chr4:123074337-123076752:-
Coord C1 exon
chr4:123076586-123076752
Coord A exon
chr4:123074991-123075150
Coord C2 exon
chr4:123074337-123074663
Length
160 bp
Sequences
Splice sites
3' ss Seq
TGTTCTGTGTATTTGTGTAGGCA
3' ss Score
7.53
5' ss Seq
AAGGTAGGA
5' ss Score
9.45
Exon sequences
Seq C1 exon
TTGCTCCAGCGACTCCTAGATGATCGAAAGGCCACAGTGGATATGCTTCAAGCAGAAGGAGGCAGAATAGCCCAGTCAGCTGAGCTGGCTGATAGAGAGAAAATCACTGGGCAGCTGGAGAGTCTTGAACGTAGATGGACTGATCTCCTCAGCAAGGCAGCAGCCAG
Seq A exon
GCAAAAACAGCTGGAAGATATCTTGGTTCTGGCTAAACAATTCCATGAGACAGCTGAGCCTATTTCTGACTTCTTATCTGTCACAGAGAAAAAGCTTGCTAACTCGGAACCTGTTGGCACTCAGACTGCCAAAATACACCAGCAGATTATTCGTCACAAG
Seq C2 exon
GCTCTAGAGGAAGAAATAGAAAACCATGCAACAGATGTGCACCAGGCAGTTAAAATTGGGCAGTCCCTCTCCTCCCTGACATGTCCGGCAGAGCAGGGCATCATGTCAGAAAAGCTAGACTCATTGCAGGCCCGATACAGTGAAATTCAAGACCGGTGCTGTCGGAAAGCATCCCTGCTCGAGCAGGCCCTGTTCAATGCTAGGCTGTTCGGGGAGGATGAGGTGGAGGTGCTCAACTGGCTGGCTGAGGTTGAGGACAAGCTCAGTACAGTGTTCGTAAAGGATTACAGACAGGATGTCCTGCAGAAACAGCATGCCGACCACCTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028649-'81-79,'81-78,82-79
Average complexity
C1
Mappability confidence:
100%=100=100%
Protein Impact





	

	
ORF disruption upon sequence exclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.022 A=0.102 C2=0.009
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0043516=Spectrin=FE(51.4=100)

							
A:
PF0043516=Spectrin=PD(5.6=11.1),PF0043516=Spectrin=PU(41.1=81.5)

							
C2:
PF0043516=Spectrin=PD(57.0=56.0),PF0043516=Spectrin=PU(40.6=39.4)

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000101819





     
                   









    
Main Skipping Isoform:
NA





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000080755, ENSMUSP00000081324, ENSMUSP00000081324f2416A, ENSMUSP00000081324f6186A, ENSMUSP00000095508, ENSMUSP00000095509, ENSMUSP00000101827, ENSMUSP00000101831, ENSMUSP00000101831f2417A, ENSMUSP00000114568, ENSMUSP00000119885
          









    
Other Skipping Isoforms:
NA
  



Associated events






Other assemblies


mm10




Conservation


Human
(hg38)
chr1:39427455-39427614 
HIGH PSI
HsaEX0037153
(MACF1)
Human
(hg19)
chr1:39893127-39893286 
HIGH PSI
HsaEX0037153
(MACF1)
Rat
(rn6)
chr5:141086981-141087140 
HIGH PSI
RnoEX0051755
(Macf1)
Cow
(bosTau6)
chr3:107230114-107230273 
HIGH PSI
BtaEX6018688
(MACF1)
Chicken
(galGal4)
chr23:5376874-5377033 
HIGH PSI
GgaEX1000790
([1])
Chicken
(galGal3)
chr23:5694221-5694380 
HIGH PSI
GgaEX1000790
(MACF1)
Zebrafish
(danRer10)
chr19:35495743-35495902 
HIGH PSI
DreEX0044245
(macf1a)
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
GCAGAATAGCCCAGTCAGCTG

				
R: 
GTATCGGGCCTGCAATGAGTC

				
Band lengths: 
244-404

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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