BtaEX6021152 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000006828 | RAPGEF3
Description
Rap guanine nucleotide exchange factor (GEF) 3 [Source:HGNC Symbol;Acc:HGNC:16629]
Coordinates
chr5:32705888-32707049:+
Coord C1 exon
chr5:32705888-32706034
Coord A exon
chr5:32706564-32706676
Coord C2 exon
chr5:32706961-32707049
Length
113 bp
Sequences
Splice sites
3' ss Seq
GGGTGTGGCTTGTTCTCCAGGAT
3' ss Score
7.35
5' ss Seq
TCGGTAACA
5' ss Score
5.35
Exon sequences
Seq C1 exon
GGCCTGGTGACCACCCTGCACGAGGGAGATGACTTCGGACAGCTGGCTCTGGTGAACGATGCACCTCGGGCCGCCACCATCATCCTGGGAGAAGACAACTGTCATTTTCTTCGTGTGGACAAGCAGGACTTCAACCGTATCATCAAG
Seq A exon
GATGTGGAAGCAAAGACCATGAGGCTGGAAGAACATGGCAAAGTGGTGTTAGTGCTGGAGAGAACCTCTCAGGGCACTGGCCCTTCTCGTCCCCCAACCCCAGGCAGGAATCG
Seq C2 exon
GTATACAGTGATGTCTGGCACCCCAGAGAAGATCCTAGAACTTCTGTTGGAGGCCATGCGGCCTGACTCCAGTGCTCATGACCCAACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000006828-'12-14,'12-13,14-14=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.500 C2=0.194
Domain overlap (PFAM):
C1:
PF0002724=cNMP_binding=FE(56.5=100)
A:
PF0002724=cNMP_binding=PD(2.4=5.3)
C2:
PF0061815=RasGEF_N=PU(25.5=90.3)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGACCACCCTGCACGAGG
R:
GTTGGGTCATGAGCACTGGAG
Band lengths:
227-340
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]