DreEX6081734 @ danRer10
Exon Skipping
Gene
ENSDARG00000079291 | rapgef3
Description
Rap guanine nucleotide exchange factor (GEF) 3 [Source:ZFIN;Acc:ZDB-GENE-090312-38]
Coordinates
chr23:33494013-33496319:+
Coord C1 exon
chr23:33494013-33494159
Coord A exon
chr23:33494236-33494330
Coord C2 exon
chr23:33496231-33496319
Length
95 bp
Sequences
Splice sites
3' ss Seq
TAACCTGGATTTAACTGCAGGAC
3' ss Score
6.39
5' ss Seq
CAAGTAAAT
5' ss Score
4.44
Exon sequences
Seq C1 exon
GGCTTGGTGACTACGCTTCATGAAGGAGATGATTTTGGCCAGTTGGCATTGGTGAATGACGCTCCTCGAGCCGCCACCATCATTTTAAGAGAGGACAATTGCCACTTTCTGCGGGTGGACAAGCAGGATTTTATACGCATTCTTAAG
Seq A exon
GACGTCGAGGCAAACACCGTCCGTCTGGAGGAGCATGGTAAAGTCGTTCTGGTTCTTGAGAAGAGCTCGGCGCAGGATACACCAAGCTGCAACAA
Seq C2 exon
GTACACGGTAATGTCAGGAACGCCGGAAAAGATCCTGGAGCACATGCTGGAGGCCATAAAACTGGAAACCAACGGAGCTGACTTCATAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000079291-'11-12,'11-11,13-12=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.094 C2=0.032
Domain overlap (PFAM):
C1:
PF0002724=cNMP_binding=FE(55.2=100)
A:
PF0002724=cNMP_binding=PD(2.3=6.2)
C2:
PF0061815=RasGEF_N=PU(25.9=90.3)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATTGGTGAATGACGCTCCTCG
R:
CAGCTCCGTTGGTTTCCAGTT
Band lengths:
180-275
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]