BtaINT0046286 @ bosTau6
Intron Retention
Gene
ENSBTAG00000011976 | CYP4B1
Description
Bos taurus cytochrome P450, family 4, subfamily B, polypeptide 1 (CYP4B1), mRNA. [Source:RefSeq mRNA;Acc:NM_001076202]
Coordinates
chr3:99940924-99942484:-
Coord C1 exon
chr3:99942378-99942484
Coord A exon
chr3:99941115-99942377
Coord C2 exon
chr3:99940924-99941114
Length
1263 bp
Sequences
Splice sites
5' ss Seq
TGGGTGAGT
5' ss Score
8.73
3' ss Seq
ATTGTGCTTTCCTGACCCAGGAT
3' ss Score
7.21
Exon sequences
Seq C1 exon
ACCAGGTCATCAGGGAACGGAAAGCAGCCCTCCAGGATGAGAAAGAGCGGGAGAGGATACAGAGCAAGAGGCACCTGGACTTCCTGGACATTCTCCTGGGGGCTTGG
Seq A exon
GTGAGTGCGCAGTCACCCACCCTTGCCTAGAACATGTCCCTGACGGGTCTTCAGACTGTCCTCCTGAGACAGGGCTCCCTGGACAGCAGCAGGGGCTGTTAAGCATCTTTTCCCTCTTTCTCCAGCAAATATGACCTGTTTCTTGTCCTGTGGTGCTGTGCTGTGCTCTGTCGCTCAGCTGTGTCCAACTCTGCAACCCCGTGAACTGTAGCCCGCCAGGCTCCTCTGTCAGTGGGGGATTCTCCAGGCAACAATACTAGAGTGGGTTGCCATGCCCTCCTCCAGGGGATCTTCCCAACCCAGGGCTTGAACCCAAATCTCCAGCATTGCAGGTGGATTATTTATCGTCTGAGCCACCAGGGAAGCCCAAGAATACTGGAGTGGGTAGCCTTTCCTTTCTCCAGGGGATCTTCCTGACCCAGGAATCGAACTGGAGTGTCCTGCATTGCAGGCAGATTCTTTACCAGCTGAGCTACCTGGGAAGCCCTTGTCCAATGGTATATATCAGCAAAGGCTCAGAGGTGGGAAGGTGACTGGTGTGGTGCTGGGGAGAGATGTAGTGGCAGGGAGGCTGGGTCTACATGAAACAGAAGACCAATTCCAGCCTTTGAAGACTCTGAAGAATCTTAGCAACCTAGAACCTTAGACTTTATACATTACTCATTCATTCATTCAAGGAGGTCATGGCAACCCATTACAGTACCCTTGCCTGGAGAATCTCATGGACAGAGGAGCCTGGGTGGGGCTAGAGTCCATAGGGTCACACAGAGTTGGACATGACTGAAGTGACTGAACACCCGCAGCAACATTCATTTATTCACTCATTCAACTGGTCCTTCCACTCCACAAACTCACTGAGCCCTACTCTTGGTTAGTTCTCACGCTAGGGATGCAGATGCTGAGAAAGCCCTAGCCCTGGTCTCCTGACTCTCACAGTCAGGTGGCAGGGACAGGAGGTAGATGTTGACAAAGACTTCTTCCTCCTCCAGAAGGCATTGTGTGTGTCAGGTGAGCCCAATTGATAGGACTGGGTAAAATCACAAGTCATTTGGCCAGAGGGATCAAAGAAGACTTCCTGGAGTAGGAGTCATCCAAGCTGGCCTTGGGAGAACAGATAGGAATCAAAGAGACGCTCCGAACCCCTAGTCTGAAAAGGAATTTCCCTCCCTCTCCCAGACCTTCATTTGACAGCTACTCTTGTGAGGCCTTGTTGGAACCTACCTCACATGCCTTGGGCTGTCCCATTGTGCTTTCCTGACCCAG
Seq C2 exon
GATGAAGAAGGCATCAAGCTATCAGATGAAGACCTCCGGGCCGAGGTGGACACATTCATGTTCGAAGGCCATGACACCACCACCAGCGCCATCTCATGGGTTCTCTACTGCATGTCCCTGTACCCCGAGCACCAGCGTCGTTGTCGGGAAGAGATTCAGGAGATTCTTGGGGACCGGGACACTTTGAAGTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000011976:ENSBTAT00000015892:7
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.139 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006717=p450=FE(7.7=100)
A:
NA
C2:
PF0006717=p450=FE(13.8=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCAGGTCATCAGGGAACGGAA
R:
ACTTCAAAGTGTCCCGGTCCC
Band lengths:
296-1559
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]