Special

DmeEX0004291 @ dm6

Exon Skipping

Gene
FBgn0085421 | Epac
Description
The gene Exchange protein directly activated by cAMP is referred to in FlyBase by the symbol DmelEpac (CG34392, FBgn0085421). It is a protein_coding_gene from Dmel. It has 5 annotated transcripts and 5 polypeptides (4 unique). Gene sequence location is 2R:6759715..6798941. Its molecular function is described by: guanyl-nucleotide exchange factor activity. It is involved in the biological process described with: regulation of Rap protein signal transduction. 20 alleles are reported. No phenotypic data is available. The phenotypic classes of alleles include: fertile; viable. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 12-18 embryonic stages, at stages throughout the pupal period.
Coordinates
chr2R:6773640-6792410:-
Coord C1 exon
chr2R:6792316-6792410
Coord A exon
chr2R:6776350-6776522
Coord C2 exon
chr2R:6773640-6773786
Length
173 bp
Sequences
Splice sites
3' ss Seq
TATTTTCAAATGTCGCTCAGCGC
3' ss Score
3.58
5' ss Seq
AAGGTAAAA
5' ss Score
8.38
Exon sequences
Seq C1 exon
TTTTTCGCGCCGGCGAGCAGGGACGCTTCTGGTATGCAGTGCTCGGCGGATCGCTGGAGGTGCGATACCATGCCCACGCCGATGCCGATGGCAAG
Seq A exon
CGCAATTTGAATATGCACAACTGGCCGAGTGTCCGAAATCCACAAACTGAATTCCAGTGGCACCGTGAGTGAGTGAGTGAGCGAGAGGGCGTCACGAGAGACAAGGAGGGAAAATGCTATTTCGTCGCAGTTGTACGTATAACGTGGAGCAATCAACGCGACATTATGCAAAG
Seq C2 exon
AGCGCAGTTACTCTGTGCAACCTGGGCGTGGGTGCCACATTCGGGGAGTCCGTACTGCATGACTTGCCGCGGGACAGCACCGTAGTGACGAAGACCACCTGCGAGCTGCTGCGGGTGGAGCAGCAGGACTTTCGACTCATCTGGGAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0085421_CASSETTE2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact

Alternative protein isoforms (No Ref, Alt. ATG)

No structure available
Features
Disorder rate (Iupred):
  C1=0.000 A=0.236 C2=0.000
Domain overlap (PFAM):

C1:
PF0002724=cNMP_binding=FE(33.0=100)
A:
NO
C2:
PF0002724=cNMP_binding=FE(51.1=100)


Main Inclusion Isoform:
FBtr0329849fB492


Main Skipping Isoform:
FBpp0302889


Other Inclusion Isoforms:
FBpp0302890


Other Skipping Isoforms:
NA
Associated events
Conservation
Mouse
(mm9)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
ALTERNATIVE
Primers PCR
Suggestions for RT-PCR validation
F:
GAGCAGGGACGCTTCTGGTAT
R:
TCCCAGATGAGTCGAAAGTCCT
Band lengths:
227-400
Functional annotations
There are 0 annotated functions for this event


GENOMIC CONTEXT[edit]

INCLUSION PATTERN[edit]


SPECIAL DATASETS

  • Neural diversity
  • Neurogenesis
  • Neuronal activity
  • Splicing factor regulation (brain)
  • Splicing factor regulation (SL2)