MmuEX1022023 @ mm10
Exon Skipping
Gene
ENSMUSG00000049044 | Rapgef4
Description
Rap guanine nucleotide exchange factor (GEF) 4 [Source:MGI Symbol;Acc:MGI:1917723]
Coordinates
chr2:72031033-72045248:+
Coord C1 exon
chr2:72031033-72031175
Coord A exon
chr2:72034031-72034119
Coord C2 exon
chr2:72045102-72045248
Length
89 bp
Sequences
Splice sites
3' ss Seq
CATCACTGTTTCTCTTTCAGTGT
3' ss Score
11.44
5' ss Seq
CAGGTAATC
5' ss Score
6.99
Exon sequences
Seq C1 exon
GCCGTTGGAGCGATCTAGTGAAGATGTGGACATAATTTTCACGCGGCTGAAAGGAGTTAAAGCTTTTGAGAAATTTCACCCAAACCTCCTTCGTCAGATTTGTTTATGCGGTTACTATGAGAACCTGGAAAAAGGAATCACAC
Seq A exon
TGTTTCGCCAAGGGGATATTGGAACCAACTGGTATGCTGTCCTGGCTGGGTCTTTGGATGTTAAAGTGTCTGAGACCAGCAGTCACCAG
Seq C2 exon
GATGCGGTGACCATCTGCACTCTGGGAATTGGGACAGCCTTTGGAGAGTCCATTCTGGATAACACCCCTCGCCATGCAACCATCGTTACCAGGGAGAGCAGCGAACTTCTCCGCATTGAGCAGGAGGACTTCAAGGCACTATGGGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000049044-'11-8,'11-6,12-8
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.014
Domain overlap (PFAM):
C1:
PF0002724=cNMP_binding=PU(9.8=18.4)
A:
PF0002724=cNMP_binding=FE(31.5=100)
C2:
PF0002724=cNMP_binding=FE(52.2=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Primers PCR
Suggestions for RT-PCR validation
F:
CACCCAAACCTCCTTCGTCAG
R:
TGCTCTCCCTGGTAACGATGG
Band lengths:
167-256
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types