DmeEX6006339 @ dm6
Exon Skipping
Gene
FBgn0032252 | loh
Description
The gene lonely heart is referred to in FlyBase by the symbol Dmelloh (CG6232, FBgn0032252). It is a protein_coding_gene from Dmel. It has 4 annotated transcripts and 4 polypeptides (3 unique). Gene sequence location is 2L:10442534..10450080. Its molecular function is described by: peptidase activity. It is involved in the biological process described with: positive regulation of extracellular matrix assembly; larval heart development. 25 alleles are reported. The phenotypes of these alleles manifest in: embryonic/larval heart; embryonic/larval cardial cell; embryonic/larval pericardial cell; embryonic/larval alary muscle. The phenotypic classes of alleles include: lethal - all die during P-stage; viable; lethal - all die before end of larval stage; short lived; cell shape defective. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of extremely low expression. Peak expression observed within 12-24 hour embryonic stages.
Coordinates
chr2L:10446185-10447982:+
Coord C1 exon
chr2L:10446185-10446366
Coord A exon
chr2L:10446523-10446680
Coord C2 exon
chr2L:10447781-10447982
Length
158 bp
Sequences
Splice sites
3' ss Seq
TTGTTTATAATAATTTATAGATG
3' ss Score
6.25
5' ss Seq
AAGGTTAGT
5' ss Score
8.54
Exon sequences
Seq C1 exon
TAACCTATTGACGAATAATCAGCTTACGAGTAATGCATGTGTCGGTTACTTTAAACGCTATCAACTGTGCAATGATGTGCCATGCGACGAACAATCCCCAGATTTTCGGGCCTCCCAATGCTCGGCTTACAACGATAAAGAGTTTCATGGTCATCGATATAGGTGGGAACCTTATGTCAAGG
Seq A exon
ATGACGCTGAATGCGAACTGAACTGTATGCCATTCGGAATGAAATCATTTGCCACACTAAACGAATCCGTAATTGATGGAACTCCATGTGGACATCCAGCTGAATATTTTAGATCGCAATTTTGGGAGCGCGCTGTTTGTGTTGATGGAGCTTGTAAG
Seq C2 exon
GCAGTGCAAGCCAGTGGCGAAATCGATGGATTGTATGCGCACAGTGGATCCGTGAGCTGTGGTGGATTACTTTGCCGGCCAGTTACGGGCATCTTTACCAGAAATCCTCTGCCGGAACATGCCTATATACATGTTACCACATTGCCAGTGGGTGCCTCCAATATTTCGATAACTGAACTTAAGAATAGCATCAACTTGCTGG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0032252-'4-7,'4-5,5-7=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0009014=TSP_1=PD(50.9=45.2)
A:
NO
C2:
PF059869=ADAM_spacer1=PU(34.7=61.8)
Main Inclusion Isoform:
FBpp0079619
Main Skipping Isoform:
FBpp0293074
Other Inclusion Isoforms:
FBpp0307931
Other Skipping Isoforms:
NA
Associated events
Conservation
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACAATCCCCAGATTTTCGGGC
R:
AATATTGGAGGCACCCACTGG
Band lengths:
257-415
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)