Special

DmeEX6010642 @ dm6

Exon Skipping

Gene
FBgn0020620 | RN-tre
Description
The gene Related to the N terminus of tre oncogene is referred to in FlyBase by the symbol DmelRN-tre (CG8085, FBgn0020620). It is a protein_coding_gene from Dmel. It has 6 annotated transcripts and 6 polypeptides (5 unique). Gene sequence location is 2R:13984815..13990707. Its molecular function is described by: Rab GTPase binding; GTPase activator activity. It is involved in the biological process described with: activation of GTPase activity; intracellular protein transport; regulation of GTPase activity. 11 alleles are reported. The phenotypes of these alleles manifest in: organism; multi-tissue structure; head segment; circulatory system; female germline cyst. The phenotypic classes of alleles include: melanotic mass phenotype; planar polarity defective; cell migration defective; neuroanatomy defective; viable. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-18 hour embryonic stages, in adult female stages.
Coordinates
chr2R:13988943-13990707:-
Coord C1 exon
chr2R:13990566-13990707
Coord A exon
chr2R:13989685-13989986
Coord C2 exon
chr2R:13988943-13989624
Length
302 bp
Sequences
Splice sites
3' ss Seq
CCATTTTTTTTTTATCAAAGTTT
3' ss Score
4.88
5' ss Seq
CAAGTGAGT
5' ss Score
9.1
Exon sequences
Seq C1 exon
CTGGCAACTCTGGCCCTCACACACACTCAGTCGCCCCCCAAATGTATCGGAAACATGCAGCCGAGATAAAATAGTGAAAAACTAGTTACCAAACATAACCGATCAGTGCGGATAATAAGAATTGGCAATTCAAAATTAAATG
Seq A exon
TTTCGCGCTATCGCCCAGTCAGCTCAGAAGCAGCCGCTCCCCAACTGAGAGCACAGAGAAACCAGCTCTGGCCACTTGCTCCTCCGGATCCGGCTCCTGCTCCTGTCCCCGATCCCCTCCCCCACCCGCCAAATCCTGATCCTGCTCCAAATGACTGACGGCGAACAGCAGGAGGCGCTGGTCAAGCGGGCCGAGGACGAGCGCGAGGACATCTTCCGGCGCTACGAACTCGGGCTGGATCCCAGCAATGTGGTGGACTCCTGGGAGAATCCCACCTTCGAGATCTACCACAGGACAGACAA
Seq C2 exon
ATATGGCTTCTTGCACGACTCGCGGCTGCCGTCGACGCGTGATGCCCAGGAGGTGCATCGCAACAAGATTGAAATGGAGCGGGACAAAAAGTGGATGAAGATGCTAAATCAGTGGCCGCCGCCCCAGGATAAGCTGCACAAGCGCGTCTACAAGGGCATACCGGATCGCGTGCGTATGGTGGCCTGGAATAAGCTGCTCGACATCCAGCAATCGATAAACAACAATGCTGGCGTCTACCTCCGAATGCTGCAGCTGGCCAGGAAGTACTCCACGGAGACGCGACAAATCGATGCCGATGTGAATCGTCAGTTTCGCGACAATCTTGCCTTTCGCGAGCGCTACAGCGTAAAGCAGTGCTCGCTATTCAATGTGCTCAACGCCTATAGCATCTACAACTCGGAGCTGGGCTACTGCCAGGGCATGGCCTGTGTGGCTGGCGTGCTGTTGCTTTATTTGCACGAGGAGGAGGCTTTTTGGGCATTAAATACCCTGATAACAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0020620-'0-2,'0-1,4-2=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact

Alternative protein isoforms (Ref, Alt. ATG (>10 exons))

No structure available
Features
Disorder rate (Iupred):
  C1=0.275 A=0.275 C2=0.057
Domain overlap (PFAM):

C1:
NO
A:
NO
C2:
PF0056613=RabGAP-TBC=PU(81.9=75.4)


Main Inclusion Isoform:
FBpp0086731


Main Skipping Isoform:
NA


Other Inclusion Isoforms:
FBpp0086729, FBpp0086730, FBpp0303476, FBpp0303477


Other Skipping Isoforms:
NA
Associated events
Conservation
Primers PCR
Suggestions for RT-PCR validation
F:
CGGAAACATGCAGCCGAGATA
R:
CTGCAGCATTCGGAGGTAGAC
Band lengths:
348-650
Functional annotations
There are 0 annotated functions for this event


GENOMIC CONTEXT[edit]

INCLUSION PATTERN[edit]


SPECIAL DATASETS

  • Neural diversity
  • Neurogenesis
  • Neuronal activity
  • Splicing factor regulation (brain)
  • Splicing factor regulation (SL2)