DreEX0012150 @ danRer10
Exon Skipping
Gene
ENSDARG00000079388 | agrn
Description
agrin [Source:ZFIN;Acc:ZDB-GENE-030131-1033]
Coordinates
chr23:23942606-23976906:+
Coord C1 exon
chr23:23942606-23942740
Coord A exon
chr23:23964279-23964462
Coord C2 exon
chr23:23976682-23976906
Length
184 bp
Sequences
Splice sites
3' ss Seq
TTGTATATCGGTCTCCACAGATT
3' ss Score
9.45
5' ss Seq
ACAGTAAGT
5' ss Score
9.49
Exon sequences
Seq C1 exon
GCCCGACCTGCGCTGATGCCCACAATCCCTGCGACCCCAATAAATGCCACCCGCCATCACGGTGCCAGGTCCTTCCAGAGGGAGGTTACAAATGCGAATGTCCGATGGGACGTGAAGGCAGGCACTGTGAAAAAG
Seq A exon
ATTACTTTGATGGGAATTCCAATCCTGAAAGCTGATAATGCCCTTTCGTCCACTGACGTGTCCATGTATCCTAATCATCCCTGCTCCAAAGACGTTTGTGAGAATGGAGGCCGCTGCAACCCCCAGCTGAACAGCTATGAGTGCGCCTGCCGCCACGGATTCTCCGGACACCACTGCCAGGACA
Seq C2 exon
CGAGAAAGCCCTGCTGGTGAATAAATTCGAGCTGAGCATCAGGACGGAGGCTACGCATGGTCTGATTTTGTGGAGCGGAAAGGGAGTTGAGCGCTCTGACTACATCGCCCTCGCCATTGTGGATGGAAGAGTACAGATGACCTATGACCTCGGCTCCAAGCCTGTGGTGCTGCGCTCCACTGTACGGGTCAACACCAACCACTGGATACGAATTAAGGCCAGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000079388_MULTIEX2-5/8=C1-C2
Average complexity
C3
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.002
Domain overlap (PFAM):
C1:
PF0000822=EGF=PD(6.2=4.3),PF0000822=EGF=WD(100=69.6)
A:
PF0005418=Laminin_G_1=PD(6.1=12.9),PF0000822=EGF=WD(100=50.0)
C2:
PF0005418=Laminin_G_1=PU(47.0=81.6)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AATAAATGCCACCCGCCATCA
R:
GGAGCCGAGGTCATAGGTCAT
Band lengths:
254-438
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]