DreEX0017622 @ danRer10
Exon Skipping
Gene
ENSDARG00000075883 | bub3
Description
BUB3 mitotic checkpoint protein [Source:ZFIN;Acc:ZDB-GENE-041010-210]
Coordinates
chr12:49195989-49197913:-
Coord C1 exon
chr12:49197588-49197913
Coord A exon
chr12:49196192-49196386
Coord C2 exon
chr12:49195989-49196058
Length
195 bp
Sequences
Splice sites
3' ss Seq
GTGTGTGTGTGTGCGTGCAGATG
3' ss Score
9.51
5' ss Seq
TCTGTAGGG
5' ss Score
-1.81
Exon sequences
Seq C1 exon
GAGGGGAGTCGCGGCTGCTCCTCTCCGCCACTCGGTCTCTCCGGTTAACGGAAATATAAACTCACTAATCCTGAAACAGATTTAATCCATACCTGAGCTGTCGATCAAACGGCTGATGCGCCGCCGGAAACACGTCACTTCTCGCGGTGATTTGTTGTTTCGTGTGCTCGTTCGCGCCAGGAAGTTGATGCGCGTAACCAGTCAGCAGTAGAGACCGTTGGACGGAGTTTTGAAACGGGCCAATGAGAGCAGAGCTCTGCTGTGAACGCAGCCAATCAAATTGAGCCCAGATGAGCGGCAGAGCAGCAGCAGAGAACAGCGGCACG
Seq A exon
ATGACCGGAGCGAATGAGTTTAAGCTGGCCCAGGGCCCGGAGGACAGCGTCTCGGCAGTGAAGTTCAGCCCGTCCTCCTCTCAGTTCCTGCTGGTGTCCTCTTGGGACGGCTCCGTGCGGCTCTACGACGCCAGCGCCAACAGCATGAGGATGAAGTACCAACACCTGGCGCCCGTGCTGGACTGCGCTTTCTCT
Seq C2 exon
GACCCAACCCATGCGTGGAGCGGTGGTCTGGACAGTCAGCTGAAGACTCATGACCTGAACACTGATCAGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000075883-'0-2,'0-1,3-2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.958 A=0.152 C2=0.250
Domain overlap (PFAM):
C1:
NO
A:
PF0040027=WD40=WD(100=55.4)
C2:
PF0040027=WD40=PU(5.3=8.3)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCGGAAACACGTCACTTCTCG
R:
TTCAGCTGACTGTCCAGACCA
Band lengths:
247-442
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]