DreEX0083723 @ danRer10
Exon Skipping
Gene
ENSDARG00000027501 | usp20
Description
ubiquitin specific peptidase 20 [Source:ZFIN;Acc:ZDB-GENE-040426-1219]
Coordinates
chr5:32702581-32707670:+
Coord C1 exon
chr5:32702581-32702683
Coord A exon
chr5:32704147-32704216
Coord C2 exon
chr5:32707238-32707670
Length
70 bp
Sequences
Splice sites
3' ss Seq
ATACTCTTTACCCTTCCCAGGTC
3' ss Score
8.4
5' ss Seq
CTGGTAGGC
5' ss Score
6.99
Exon sequences
Seq C1 exon
GATCCTGTTTCGCAGACTACATGTTACCCATTGAAGGCGGTGCCTATCGCAGTGGCAGATGAGGAGGGCTCAGAGTCTGAGGAGGATGAGCTCAAACCTAGAG
Seq A exon
GTCTGACAGGGATGAAAAACATTGGAAACTCTTGTTATATGAATGCTGCACTGCAGGCCTTGTCCAACTG
Seq C2 exon
GACACTCAGGAGTTTTTGCGATGTCTGATGGACCAGCTTCACGAGGAGCTGAAGGAGCCTTTGTTTGACTGCAGCGGTGGCATCTCTGAAGTCGAGCCTGACCTCAGTCTGGACAGCTGTAATCTTGTGGACGGAGACCGAAGCCCCTCTGAAGACGAATTCCTGTCGTGTGACTCTGGATCAGGAAGCGAAAGGGGAGATGGAGAGCGTGCAGGAGGAGAAGCAGAGCTCCTCATTCAAGATGAATGTGTGGCAGTGAGAGGAACCGGAGGCATCTCGGAGAAAGAGAGGCTGAAGGAGAGAAGAGGAGAAGAGAGGACACGTGAAATGGATGAGGATGCAGATGTGGACACAGCTGCACAAGACGGACAGGCTGAGAGAGAAACTGAAACAGCGACACCAGCCACAGCTGTGCCGGCACCAGGAAACACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000027501_MULTIEX1-4/22=3-7
Average complexity
C3
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.429 A=0.042 C2=0.593
Domain overlap (PFAM):
C1:
NO
A:
PF0044324=UCH=PU(16.4=91.7)
C2:
PF0044324=UCH=PD(34.3=46.5)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGTTACCCATTGAAGGCGGTG
R:
GCTCGACTTCAGAGATGCCAC
Band lengths:
179-249
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]