DreEX6002506 @ danRer10
Exon Skipping
Gene
ENSDARG00000026855 | cacna2d4a
Description
calcium channel, voltage-dependent, alpha 2/delta subunit 4a [Source:ZFIN;Acc:ZDB-GENE-041210-213]
Coordinates
chr4:20569276-20569828:-
Coord C1 exon
chr4:20569712-20569828
Coord A exon
chr4:20569529-20569588
Coord C2 exon
chr4:20569276-20569438
Length
60 bp
Sequences
Splice sites
3' ss Seq
TTATTTCATGTCATTTATAGAGA
3' ss Score
6.81
5' ss Seq
GAGGTGAGT
5' ss Score
10.03
Exon sequences
Seq C1 exon
AAATATAAAGAAGTGGAACCCATCGTCAAGATGGAGGAGATCGATGGTTTAAAAATGGTGAAGGAATTTGCTGAGGAAATGGAGAACATGCTGGGCAGAAAAATGAAATCAGTTAAG
Seq A exon
AGAATGGCAGAGACAGCAGAAGACGCAGATTTTTATCATGAATTTAATGCCACATTAGAG
Seq C2 exon
TTTGACTACTACAATTCCATGTTGATTAACACATTAAATGAAGATGGAAACAACATAGAGCTGGGGGGTGAATTTCCTTTGGAGGAGAATGAACACTTCAACAATCTGCCTGTCAATACGCAGCAAAGCGTCGTACAAGTGCCCACAAATGTTTATAACAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000026855-'3-2,'3-1,4-2=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.236
Domain overlap (PFAM):
C1:
NO
A:
PF083996=VWA_N=PU(12.0=66.7)
C2:
PF083996=VWA_N=FE(46.2=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCTGAGGAAATGGAGAACATGC
R:
CCTCCAAAGGAAATTCACCCCC
Band lengths:
133-193
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]