DreEX6011332 @ danRer10
Exon Skipping
Gene
ENSDARG00000035505 | nelfb
Description
negative elongation factor complex member B [Source:ZFIN;Acc:ZDB-GENE-040426-765]
Coordinates
chr5:28948830-28951094:-
Coord C1 exon
chr5:28950953-28951094
Coord A exon
chr5:28950733-28950841
Coord C2 exon
chr5:28948830-28949354
Length
109 bp
Sequences
Splice sites
3' ss Seq
TGGTTTGTTTTGATTTGCAGTAA
3' ss Score
6.99
5' ss Seq
CAGGTATTT
5' ss Score
7.51
Exon sequences
Seq C1 exon
TGGATACATATAATGACATGGCATTCGGAGATATATTTCTCCATCTGCTCACTGGTCATCTGACTCTTCTCTCTGATGAGTTTGGAACAGAGGAGTTTTGTACTGCTGTCTTTGATAACTTCCTACTCACGTCTTTCTCAAG
Seq A exon
TAAGGAGAACGTTCACAGGCATTGCCTTCGCTTGCTGAATCATCTCCATCAGAAGGTGCTGCCGACTTACATGGAAACCTTGATGAAGACGCTTGAACCACCAAAACAG
Seq C2 exon
AGCAGTGAGTCGGTGAAGGAGCTGTACAACTTGCTGAAGGAGAAGCTGGAGGCCTCTAAGAAAAGCCCCCCGCAACCAGAGGAAGCCCCTTCTCTAGATCTCGGCCTTCATCCTGTTACTGTGCCGCCCACTCCCTCCACCCCCACAACCCCTGTCTAATGGACATGGGACATCCCGCACACTAAACAGCAGCAGTGTTTGAATGGAGGTGTACGGCATGCCTAATTGTGTAAAGACTGAACTATGTGTCAAATGGAACTGTGCTGTTCATGTGACATAGTTATGTTATTTTTCTCCTTTTTTTTCTCCCCTCTTTTATAAAGGTCATTTTAAAAGTGTTTTCCATTTTTGCAAATCCATGGGCTCGGCTCTATTTCAATTTCTACACTTCTTTTAAGAAAATGTAGTCCAGTTGTTGAATGAGTTCTGTAGTATATTATGTACAGCATCTCTCATGGTTGATGGGATTTGCTTTGGTTTGTGTCAGTGGCAAGCCCTGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000035505-'14-18,'14-17,15-18=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref, Alt. Stop)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.189 C2=0.687
Domain overlap (PFAM):
C1:
PF062098=COBRA1=FE(10.0=100)
A:
PF062098=COBRA1=FE(7.6=100)
C2:
PF062098=COBRA1=PD(9.4=83.0)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGCTCACTGGTCATCTGACTCT
R:
ATTAGACAGGGGTTGTGGGGG
Band lengths:
257-366
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]