MmuEX6099421 @ mm10
Exon Skipping
Gene
ENSMUSG00000013465 | Nelfb
Description
negative elongation factor complex member B [Source:MGI Symbol;Acc:MGI:1931035]
Coordinates
chr2:25199712-25201186:-
Coord C1 exon
chr2:25201045-25201186
Coord A exon
chr2:25200659-25200767
Coord C2 exon
chr2:25199712-25200466
Length
109 bp
Sequences
Splice sites
3' ss Seq
GCTCCACTGATCTCTTCCAGGAA
3' ss Score
9.02
5' ss Seq
CAGGTGGGT
5' ss Score
8.56
Exon sequences
Seq C1 exon
TGGAAACGTTTGGTGACCTGGCCTTCAGTGACATCTTTCTCCACCTGCTCACTGGGAGCCTGGTACTGCTTGCTGATGAATTTGCCCTGGAGGATTTCTGCAGCAGCCTCTTTGATGGCTTCTTCCTCACTGCCTCTCCCAG
Seq A exon
GAAGGAGAATGTGCACCGTCATGTGCTGAGGCTTCTCCTCCACTTGCATGCAAGAGTGGCCCCTTCCAAGCTGGAGGCATTGCAGAAGGCTCTGGAGCCCACAGGCCAG
Seq C2 exon
AGCGGGGAGGCCGTGAAGGAGCTCTACTCCCAGCTTGGTGAGAAGTTGGAGCAGTTAGACCATCGAAAGCCCAGTCCTACCCAAGCTGCAGAGACACCAGCACTGGATCTGCCTCTTCCCAGCGTGCCTGCTCCAGCCACACTGTGAGGCTGTGGAGAACTGAAACTGTTTGACAGTGGTGCCCTATGGAAGGGCACCTAGTTGGCTAGGGGTGTAGGCTGAGCTGAGAGGGGTGTTCTTTGGCAAGGTTCTCCCTCCTCTAGTTTCTCCCAGGAGCTGGTCCCAAAAGGTCCTCCCTCAGTGGTCTACTTGCATATCATAGGGGGCCCTTGATGTCATGGTGGGACTTGGCCCCTGCTTGACCCTGGCAGAGGTGGATGGTGATTGGGATATAGGAAAAGCAGTGGGCAGAGGCCTGACTGACCTGATATTCAATCTGCACAAAGTCCAAGGTATGCTAGGTTCACCAAAGTAGATTTGCTGGACCTGATCCCCAAGCA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000013465-'22-32,'22-30,26-32
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref, Alt. Stop)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.024 C2=0.500
Domain overlap (PFAM):
C1:
PF062098=COBRA1=FE(32.6=100)
A:
PF062098=COBRA1=FE(7.6=100)
C2:
PF062098=COBRA1=PD(9.9=95.9)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GATGGCTTCTTCCTCACTGCC
R:
GCCTACACCCCTAGCCAACTA
Band lengths:
248-357
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types