DreEX6020125 @ danRer10
Exon Skipping
Gene
ENSDARG00000075924 | RAPGEF4 (1 of 2)
Description
Rap guanine nucleotide exchange factor (GEF) 4 [Source:HGNC Symbol;Acc:HGNC:16626]
Coordinates
chr6:6693652-6705537:+
Coord C1 exon
chr6:6693652-6694061
Coord A exon
chr6:6702706-6702848
Coord C2 exon
chr6:6705449-6705537
Length
143 bp
Sequences
Splice sites
3' ss Seq
TGTCTCGCTGTGGGTTTTAGGCC
3' ss Score
8.58
5' ss Seq
CCTGTAAGT
5' ss Score
7.52
Exon sequences
Seq C1 exon
CTGGAAGACAGGACTCCTGACAGCGCTCCCCGGTGGAGATGCAGCATCCGCGGTGACACTGCAGCCTCAGCACCGCAGATCTCCCGCTGACACTACCCTATTTCTCCATCAAGTGGGATTTGATTTCATCGCTCGGTGCATGTGTAGAAACGCGTTACTGCACGCTGTCTGCTGTCGTGCGTGTGTGGACGTGGTGACGATGCGTCCACGCCGCGGCGATCCACGCAGAGGCGGGGAGAGGAGGGGGGTTGAGCCCTGCTGCTGCTGATGCTGATGAAGCGACCAAGTCGACGAGAACCGCGATCCGAGACGCACGATCCAGCACACCGCTTTTAGCCGAAGATGGTAGCGGTGCACGCCTCTGCGCACTCCGTGGCCTCTGCCGAGTGGATCATCTGCCTGGATAAGAG
Seq A exon
GCCAGCTGAACGCTCGGGAGAAGACGTGGACATCATTCTGGCTCGGCTGAAGGGTGTGAAGGCCTTCGAGAAGTTTCATCCTAACCTGCTGCAGCAGATCTGCATGTGTGGCTTTTATGAGTACCTAGAGAAAGGCATCACCT
Seq C2 exon
TGTATCGACAGGGTGACATCGGCACCAGCTGGTACGCCGTTCTGTCTGGATCTCTGGACGTCAAAGTGTCTGAAACAGCCAACCATCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000075924-'0-1,'0-0,2-1=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF0002724=cNMP_binding=PU(9.8=18.4)
C2:
PF0002724=cNMP_binding=FE(31.5=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Primers PCR
Suggestions for RT-PCR validation
F:
GCTGCTGCTGATGCTGATGAA
R:
CTGATGGTTGGCTGTTTCAGAC
Band lengths:
242-385
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]