DreEX6023714 @ danRer10
Exon Skipping
Gene
ENSDARG00000011163 | sema3fa
Description
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fa [Source:ZFIN;Acc:ZDB-GENE-050513-1]
Coordinates
chr6:42914215-42918238:+
Coord C1 exon
chr6:42914215-42914282
Coord A exon
chr6:42914391-42914524
Coord C2 exon
chr6:42916495-42918238
Length
134 bp
Sequences
Splice sites
3' ss Seq
TTTGTTTTCATTCCCATCAGCCA
3' ss Score
6.47
5' ss Seq
GAGGTACAG
5' ss Score
6.62
Exon sequences
Seq C1 exon
GCGGAGTCGAAGGCAAGACGTGAAGTATGGAAACCCTATAAGGCAATGCAGAGGCTACAACTCGAATG
Seq A exon
CCAATAAGAACACATTGGAGACAGTGCAGTATGGAGTAGAGGGAAGCACAGCCTTTTTGGAGTGTCAGGCCAGATCTCCTCATGTGTCCATCAAGTGGCATTTCCAGAAGGAGAACAGCGACCGGAGAAGAGAG
Seq C2 exon
ATCCGTTCTGACGGGCGTGTGGTTCGCACGGAACAGGGCCTCCTCCTGCGCTCCCTCCAGCTGTCTGACAGCGGTGTTTACCAGTGCACCTCAACTGAGAAGAACTTCAAACACACCCTTGTCAAACTCCAGCTCGTGGTTCTCTCCGCCCACACAGTCAACAGCATCCAGACTGAAAGCGGCGCCCCGGCCGCCCCACCCCTTCAGTCCAGCGCCTGGACGCCCAGCGCAGAACAATACAAAGACCTCCTGACCATCCTCAGCCAACCTGAGATGGGCCTCATCAACCAGTACTGCCAAGACTACTGGCAGATAGGGGACGACCCCATGGCCCACAAGAAGAAGGACCTCAAGGAGCTCCAGAAGGACCTGCGGAAGCCTCGAAATAGGAGACACCACCAGGAGCAGAGCAGCATGGCTGAGACATGAGGAAACTTGCATAATAATCCACCAACAACCATCATGGCCGAGGAGAACATAAACAATACGTTTCTCTTGAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000011163-'20-24,'20-23,21-24=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref, Alt. Stop)
No structure available
Features
Disorder rate (Iupred):
C1=0.167 A=0.111 C2=0.373
Domain overlap (PFAM):
C1:
PF0143720=PSI=PD(35.7=83.3),PF0768612=V-set=PU(0.1=0.0)
A:
PF0768612=V-set=PU(47.8=97.8)
C2:
PF0768612=V-set=PD(50.0=32.2)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTCGAAGGCAAGACGTGAAGT
R:
CGCTTTCAGTCTGGATGCTGT
Band lengths:
244-378
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]