DreEX6023716 @ danRer10
Exon Skipping
Gene
ENSDARG00000011163 | sema3fa
Description
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fa [Source:ZFIN;Acc:ZDB-GENE-050513-1]
Coordinates
chr6:42910925-42912044:+
Coord C1 exon
chr6:42910925-42911069
Coord A exon
chr6:42911153-42911375
Coord C2 exon
chr6:42911956-42912044
Length
223 bp
Sequences
Splice sites
3' ss Seq
TGCTATCCCTCTAATCTTAGTGT
3' ss Score
6.82
5' ss Seq
CAGGTGAAC
5' ss Score
5.56
Exon sequences
Seq C1 exon
GTCTGTGTTTAAAGGCTCAGCAGTATGTGTTTATTCAATGGCTGATGTGCGCATGGTCTTCAATGGGCCTTTCGCTCATAAAGAAGGCCCAAACTACCAATGGGTTGCATACCAGGGCAAAATCCCATACCCTCGCCCAGGAACA
Seq A exon
TGTCCAGGAGGGACGTTTACCCCCAACATGAAGTCTACTAAAGATTACCCAGATGAGGTGATCAACTTCATGCGCACACATCCCACCATGTTCAATGCAGTTTATCCAGTCCACAAGCGCCCCCTGGTGGTACGAACCAACGTGGATTATGAGTTCACCACCATCACTGTGGACCAAGTGGCTGCAGCTGATGGCAACTATGAAGTTCTGTTCTTAGGAACAG
Seq C2 exon
ACAGGGGAACAGTTCAGAAAGTGATTGTCCTGCCCAGAGATGATCTACAGACTGAAGAGCTGGTTTTGGAGGAGGTGGAGGTCTTCAGA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000011163-'15-18,'15-17,16-18=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.020 A=0.120 C2=0.000
Domain overlap (PFAM):
C1:
PF0140314=Sema=FE(10.2=100)
A:
PF0140314=Sema=FE(15.7=100)
C2:
PF0140314=Sema=FE(6.1=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCTGTGTTTAAAGGCTCAGCAGT
R:
GAAGACCTCCACCTCCTCCAA
Band lengths:
230-453
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]