RnoEX6037499 @ rn6
Exon Skipping
Gene
ENSRNOG00000017704 | Sema3f
Description
semaphorin 3F [Source:RGD Symbol;Acc:1308514]
Coordinates
chr8:116443721-116446140:-
Coord C1 exon
chr8:116445996-116446140
Coord A exon
chr8:116444360-116444582
Coord C2 exon
chr8:116443721-116443809
Length
223 bp
Sequences
Splice sites
3' ss Seq
CCCTCGCCGTCTGCTTGCAGTGC
3' ss Score
8.51
5' ss Seq
CAGGTACCC
5' ss Score
8.63
Exon sequences
Seq C1 exon
CTCTGTGTTCCGAGGATCTGCAGTGTGTGTCTACTCTATGGCTGACATCCGCATGGTCTTCAATGGGCCTTTTGCTCACAAGGAGGGCCCCAACTACCAGTGGATGCCTTTCTCAGGAAAGATGCCGTATCCCCGGCCTGGCACA
Seq A exon
TGCCCTGGTGGAACCTTTACGCCATCGATGAAGTCCACCAAGGACTATCCTGATGAAGTGATCAACTTCATGCGTACTCATCCACTCATGTACCAGGCCGTCTACCCGCTGCAGCGGCGCCCCCTGGTGGTCCGTACAGGCGCTCCCTACCGCCTCACCACTGTCGCTGTGGACCAGGTGGATGCAGCTGATGGACGCTATGAGGTGCTTTTCCTGGGCACAG
Seq C2 exon
ACCGCGGGACAGTGCAGAAGGTCATCGTGCTGCCCAAGGATGATCAGGAGGTGGAAGAGCTCATGTTAGAGGAGGTGGAGGTCTTCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000017704-'21-19,'21-16,23-19=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.041 A=0.147 C2=0.067
Domain overlap (PFAM):
C1:
PF0140314=Sema=FE(10.1=100)
A:
PF0140314=Sema=FE(15.6=100)
C2:
PF0140314=Sema=FE(6.1=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTGTTCCGAGGATCTGCAGTG
R:
TTGAAGACCTCCACCTCCTCT
Band lengths:
229-452
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]