DreEX6056041 @ danRer10
Exon Skipping
Gene
ENSDARG00000041864 | capn3a
Description
calpain 3a, (p94) [Source:ZFIN;Acc:ZDB-GENE-040912-97]
Coordinates
chr17:45344961-45352770:+
Coord C1 exon
chr17:45344961-45345169
Coord A exon
chr17:45350086-45350122
Coord C2 exon
chr17:45352657-45352770
Length
37 bp
Sequences
Splice sites
3' ss Seq
GTTTGTTGGTTTCTTCTCAGGGA
3' ss Score
8
5' ss Seq
GTGGTAAGT
5' ss Score
10.36
Exon sequences
Seq C1 exon
ATGCATGGAAATAAGCAGCACATGCAGAAGGAGTTCTTCATGTCTACCACCGCTAAGGCTCGGTCCAGGGCCTACATTAACCTGCGCGAGGTCACCCAGCGTTTCCGTCTGAGTCCCGGAGAGTACGTCATCATTCCCTCTTCTTACGAGCCCCATCAGGAAGGCGAGTTCATCCTCCGCGTCTTCTCTGAAAAGAGAAATACTTCTGA
Seq A exon
GGAGATAGAGAACAGGATTGAAGCTGATCATCCAGTG
Seq C2 exon
CCTTCTCCGGCTTCAACTGGGGAGGAGAGCGAAGAGGACCAACAGTTCCTGTCCATTTTCCAGCACATCGCGGGGGACGTGAGTACTGGGGAGGCAGTGAGCGCTTGGGGGAGT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000041864-'16-16,'16-15,18-16=AN
Average complexity
A_S
Mappability confidence:
100%=80=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.173 A=1.000 C2=0.289
Domain overlap (PFAM):
C1:
PF0106717=Calpain_III=FE(44.5=100)
A:
PF0106717=Calpain_III=PD(0.1=0.0)
C2:
PF138331=EF-hand_8=PU(0.1=0.0)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTCCCTCTTCTTACGAGCCCC
R:
ACTGTTGGTCCTCTTCGCTCT
Band lengths:
122-159
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]