DreEX6056048 @ danRer10
Exon Skipping
Gene
ENSDARG00000041864 | capn3a
Description
calpain 3a, (p94) [Source:ZFIN;Acc:ZDB-GENE-040912-97]
Coordinates
chr17:45330946-45334986:+
Coord C1 exon
chr17:45330946-45331064
Coord A exon
chr17:45331132-45331265
Coord C2 exon
chr17:45334818-45334986
Length
134 bp
Sequences
Splice sites
3' ss Seq
GAGTTGATTTCTGTTCTCAGTTC
3' ss Score
6.47
5' ss Seq
TAAGTGAGT
5' ss Score
6.43
Exon sequences
Seq C1 exon
GTGACTGCTGGTTGCTTGCAGCCATTGCCTGTCTGACTCTGAATGACAAGCTGCTGTATCGTGTTATTCCTCAAGAGCAGAGCTTCTCCGAACAATATGCAGGAATTTTCCATTTCCAG
Seq A exon
TTCTGGCGATATGGGGATTGGGTGGATGTTGTGGTTGATGACCGCATTCCCACCTTCAACAACCAACTGGTGTTCACCAAGTCTGCTGAGAGGAACGAGTTCTGGAGCGCTCTTCTGGAGAAGGCTTACGCTAA
Seq C2 exon
GCTTCATGGGTCCTATGAGGCCCTGAAGGGTGGAAACACAGCCGAGGGAATGGAGGACTTCACTGGTGGAGTGACTGAATTCTACGAGATGAAGGAGGCGCCCAAAGAGCTGTATAAGATCATGCAGAAAGCTCTAGAGAGAGGCTCACTCATGGGCTGCTCCATTGAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000041864-'5-4,'5-2,6-4=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.002
Domain overlap (PFAM):
C1:
PF0064816=Peptidase_C2=FE(12.8=100)
A:
PF0064816=Peptidase_C2=FE(14.5=100)
C2:
PF0064816=Peptidase_C2=FE(18.4=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCCATTGCCTGTCTGACTCTG
R:
CCCATGAGTGAGCCTCTCTCT
Band lengths:
255-389
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]