DreEX6083482 @ danRer10
Exon Skipping
Gene
ENSDARG00000019231 | spna2
Description
spectrin alpha 2 [Source:ZFIN;Acc:ZDB-GENE-051113-60]
Coordinates
chr21:13091530-13092164:+
Coord C1 exon
chr21:13091530-13091727
Coord A exon
chr21:13091823-13091969
Coord C2 exon
chr21:13092061-13092164
Length
147 bp
Sequences
Splice sites
3' ss Seq
TCCTTTTTTGTCTCCCTCAGTTG
3' ss Score
10.89
5' ss Seq
CAGGTCAGA
5' ss Score
7.41
Exon sequences
Seq C1 exon
GAACACCGCACTGAGATCGACGCTCGTGCAGGAACCTTCCTGGCTTTTGAGCAGTTTGGACAGCAGCTGCTCGCTCGTGGGCATTACGCAAGCCCTGAGATCAAACAGAAGCTGGAGGCTCTTGACCGGGAGCGTGCTGACCTGGAGAAGGCCTGGGTTCAGCGCAGGATGATGCTGGACCAGTGCCTGGAGCTGCAG
Seq A exon
TTGTTTAACCGTGACTGTGAGCAGGCTGAGAACTGGATGGCTGCCCGCGAAGCCTTCTTAGCCAGCGATGACAAGGGAGATTCTCTGGACAGTGTGGAGGCGCTCATTAAGAAACACGAGGACTTTGATAAGGCTATCAATGTGCAG
Seq C2 exon
GAAGAAAAGATTGCTGCACTGCAGTCTTTTGCTGACCAGCTCATCAGCGCTGACCACTATGCCAAGCCTGAGATCTTCAAACGACGCAGTGAGGTCTTGGACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000019231-'40-47,'40-46,42-47=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.030 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=PD(57.1=90.9),PF0043516=Spectrin=PU(2.9=4.5)
A:
PF0043516=Spectrin=FE(45.7=100)
C2:
PF0043516=Spectrin=FE(32.4=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCTTCCTGGCTTTTGAGCAGT
R:
CCTCACTGCGTCGTTTGAAGA
Band lengths:
258-405
Functional annotations
There are 1 annotated functions for this event
PMID: 9712874
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: classical fluorescence spectroscopy, cleavage reaction, glutathione s tranferase tag, western blot. ELM ID: ELMI001910; ELM sequence: DSLDS; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]