DreEX6096576 @ danRer10
Exon Skipping
Gene
ENSDARG00000067859 | scospondin
Description
subcommissural organ spondin [Source:ZFIN;Acc:ZDB-GENE-051114-1]
Coordinates
chr24:40258793-40266074:+
Coord C1 exon
chr24:40258793-40258989
Coord A exon
chr24:40262627-40262754
Coord C2 exon
chr24:40265929-40266074
Length
128 bp
Sequences
Splice sites
3' ss Seq
ATTGTGCTGCTGTGTGTCAGGTT
3' ss Score
7.82
5' ss Seq
GAGGTGAGT
5' ss Score
10.03
Exon sequences
Seq C1 exon
TGTGTGTGTGAACGGCTCTCTGGTGTGTACGGATCATGTGTGTCCGCTGTACGGCTCATGGGGATCGTGGAGTGTGTGTTCGGTGTCGTGCGGGACAGGTCAGAGGACACGTGTGCGCTCCTGCACACACACCAGTGTAGGATTAAGCTGTACACACACCATCCAGTCCCAGAACTGCTCACTGAGCACCTGTCCAG
Seq A exon
GTTGTCCTGCTGGTCAGATCTTCAGCTCGTGTGCTGGATCATGTCCCTTCAGCTGTGAGGATCTGTGGCCGGAAAACCAGTGTGTGCCGCTGGACTGCAGTGCTGGGTGCAGATGCCCGTCTGGAGAG
Seq C2 exon
GTGATGCTCAACGGGACGTGTGTTTCTCAATCCCAGTGTCCGTGTTCAGTGTTCTCGCTCCTGCTGGAGTCTACAAACAGCACTCTGGATTTCCAGGATCACGAGGTTCCAGCCGGGACCGTCATCCCCAACTCCTGCAACTCATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000067859-'104-110,'104-109,106-110=AN
Average complexity
A_C2
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF053758=Pacifastin_I=PD(59.4=28.4),PF0009014=TSP_1=WD(100=70.1)
A:
PF0182612=TIL=PU(74.5=95.3)
C2:
PF0182612=TIL=PD(21.8=24.5)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGGATCGTGGAGTGTGTGTTC
R:
TGGAACCTCGTGATCCTGGAA
Band lengths:
248-376
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]