DreEX6096622 @ danRer10
Exon Skipping
Gene
ENSDARG00000067859 | scospondin
Description
subcommissural organ spondin [Source:ZFIN;Acc:ZDB-GENE-051114-1]
Coordinates
chr24:40149534-40152467:+
Coord C1 exon
chr24:40149534-40149657
Coord A exon
chr24:40151146-40151318
Coord C2 exon
chr24:40152249-40152467
Length
173 bp
Sequences
Splice sites
3' ss Seq
ATGACTGTGTTGTGTCCCAGGCT
3' ss Score
7.93
5' ss Seq
AAGGTAACA
5' ss Score
8.92
Exon sequences
Seq C1 exon
GACTTTGTGGATAAGAAGCTCAGCATCATCATCGGTGCAGGGCCGTGTGCTGGGGGAGAAGCTGGATGTCTGAGAGAGATGAGCATCACTGCGCTACACACCACACTCACTGTCACTGATGCAG
Seq A exon
GCTCAGTGCTGGTGAACGGGCACAGGAATCCTCTGCCGGTGGTCACAGGAGATCTGCTGGTCAGGCGAGCGTCCTCTAGTGTCCTGCTGATTCAGGCGTTCGGTGCTCAGCTGATGTGGCATCTGGACGGGCCTTTATTATTCATCACCCTGCAGCCTGGCTTCGCTCATAAG
Seq C2 exon
GTTCGAGGGTTGTGTGGCACTCTGAGCTGGAGTCAGCATGATGATTTCACCACTCCTGAAGGTGATGTGGAGAACAGCGCCGCCTCTTTTGCTGCCAAATTCATAGTGGGCTCCTGTCCTGCACCCTCAGCGATGACGTCTGACCCCTGCGGGACGCTGACCCAGCGCAGACAGCTGGCAGAGAGAGTGTGTTCAGTCATCCACAGCAATGTCTTTCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000067859-'13-16,'13-15,14-16=AN
Average complexity
A_C1
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.027
Domain overlap (PFAM):
C1:
PF0009420=VWD=FE(27.2=100)
A:
PF0009420=VWD=FE(37.7=100)
C2:
PF0009420=VWD=PD(13.9=28.8),PF087426=C8=PU(21.9=21.9)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCAGCATCATCATCGGTGCAG
R:
GTCAGACGTCATCGCTGAGG
Band lengths:
249-422
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]