DreINT0056590 @ danRer10
Intron Retention
Gene
ENSDARG00000076025 | dgkzb
Description
diacylglycerol kinase, zeta b [Source:ZFIN;Acc:ZDB-GENE-111104-4]
Coordinates
chr25:7737023-7737260:+
Coord C1 exon
chr25:7737023-7737143
Coord A exon
chr25:7737144-7737223
Coord C2 exon
chr25:7737224-7737260
Length
80 bp
Sequences
Splice sites
5' ss Seq
ACGGTGAGG
5' ss Score
9.2
3' ss Seq
TTTGATTTTTTGATAAACAGCTC
3' ss Score
5.26
Exon sequences
Seq C1 exon
TTGGAACAGATGCATGAAGAAGGAGCGGATCTCTCGGTCCAGGACCATTTAGGTTGCACCATCCTCCATCAGGCAGTCATTTTAGGTCACAAAGAGATTGTGAAGTTTATCACTGAAAACG
Seq A exon
GTGAGGTTCGCTTTTACTTCACTCTTTGACTTTGTGTGTTTTATTTCTGATCTTTTTTTTTTTGATTTTTTGATAAACAG
Seq C2 exon
CTCCTGCCTGCATACTGGATGTGACGGAGAAATCAAC
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000076025:ENSDART00000155016:31
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.220 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF127962=Ank_2=FE(47.1=100),PF127962=Ank_2=PU(0.1=0.0)
A:
NA
C2:
PF127962=Ank_2=PD(7.1=46.2),PF127962=Ank_2=PU(15.6=92.3)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACAGATGCATGAAGAAGGAGCG
R:
TGATTTCTCCGTCACATCCAGT
Band lengths:
151-231
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]