DreINT0091820 @ danRer10
Intron Retention
Gene
ENSDARG00000028533 | macf1a
Description
microtubule-actin crosslinking factor 1a [Source:ZFIN;Acc:ZDB-GENE-030131-3606]
Coordinates
chr19:35552196-35553892:-
Coord C1 exon
chr19:35553602-35553892
Coord A exon
chr19:35552346-35553601
Coord C2 exon
chr19:35552196-35552345
Length
1256 bp
Sequences
Splice sites
5' ss Seq
GAGGTTTGC
5' ss Score
3.52
3' ss Seq
CATTCATTACGGTTATATAGGTT
3' ss Score
5.17
Exon sequences
Seq C1 exon
CAGTTACAGAACTCTCTCACTGCCCGATCCAATGAAGTCGGCAACGTGGCGTTCAATATTCAGGTGTTCATCTCTGAGCATGCTCAAGACCTGCTGCCTGATCAGAGCAGACACCTTCTTGGACAGCTGGAGCAGCTGCAGAGGGTCTTTCACCAGGCCGTTGGTCTCAATGATGCCAGAGCAGAGGCACTATTAGCCCAGCAGATGAGAGAGCATGAACGACTGAAGAAAGAACAGAAAGTGAAAGAAGAGAAAATGGAAAAAGAGAGACAAGCTACAAGGGATAGAGAG
Seq A exon
GTTTGCAAACAAACGAATAACCGCTTGAGCTGGCAGATGAATAATAAGAATATTAATTTTTCTTTTCAATGGAGACTAAGGAGAACTTGTAATGCATAAGAGCAAAACATTTGTGGTAACACTAATGATGCATGCAATACTCGTTAGCATGAATGCTGAATTTGAAAATGAAAGTAATATTCACATGCATGAGATTATAAGTATATTCTACTTGTGTGTTTAGCACCAGTATTGCTCATAATGCTAATATTTTTTTTCTCAATAATGCTTATGCAGGACTCAACAATGACGACTTTTGTCTACCGTCTACTCAAACAAGTCATTTAGAATTTTTGTTATATATTTAAATGTGGATGTGTATTTCTTTTGGCTATACAAAAACATTTAAATCTAAAAACAAGTACATTTTATGGCTAAAATGATTGTTTATTTAAAATGTAACTCTAAGCAGTACATTCTATTAATATACTTTATTATACTTAGTATTATGTACATTATAATATAATAATGTAATAAATAAAAAATAAAAAATAAATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATACATACACACACACACACACACACACACATGTACTTTATCTTCCTCTTTGGCATGTACAAAATATACCACTTAAAAATGTAATGCTTGTGAATATGCAATAAATTCTCTTCCCTTCTAACTCTAATGCATGCAAGACTATCACGGGTATCCATTTTGAGTCATATATAACATTACTTTATTCGAATCAGCGGAGTTCCTTATTGTTGAGCCCTGTTTTGTAGACTGCATTACCTGCGTGTGAATTAACATAACGTGTGTATTAACCTATCTGCCTTGCTCTTACAGATTGCCACAGAAGACACATTTGGGAAAGACTATAGATTTAAATGTAGCCTCACTGTGTAGCTTGCTTGTCATTTAATTTGATTTATTTCAGAACAAAATCCAGTTGAGATGTTTTACTATTTGTCTGTTTGTCTTTTGTTTATTTTTCTGCCTCACTGGCCTGTTTGAGAGTGTTGCTTTGTGTGATTGTGGACACATTATGCTCAACCAGTGTCAATTGGTTGAGACCTGGCATATTTGCAAGTTTGAGAGTTTGGTGGGGAAGTAATAATATAATGAACAATTGTAAAGTCTTAATGATGATCAATTTCAGATCTTCTTTTCCTCACTTTTCATACCTCATTCATTACGGTTATATAG
Seq C2 exon
GTTGTGCAGCAGCAGAAGGCTGAATGTTCTCAAAAACTGGAGAGTCTCACAATGTGGATGGCTGGTGCTGCAAGTCTTTTGGCCAGTCAGAGACCTGGAGTTGAGTCAGATGACGTGCACACATTACAGGAGAAGCAAAGAGCGCTTAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000028533:ENSDART00000146394:38
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.392 A=NA C2=0.526
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=PD(58.0=67.0)
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCGATCCAATGAAGTCGGCAA
R:
CAAAAGACTTGCAGCACCAGC
Band lengths:
349-1605
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]